In this paper for the first time EngA GTP-binding protein of M. tuberculosis H 37 Rv was functionally characterized for its GTPase and GTP-hydrolyzing activity. GTPases such as era, obg, lepA, and FtsZ are vital for growth and development and specifically cellular functions of bacteria, in...
DNA结合蛋白质类大肠杆菌肽链终止蛋白质酪氨酸磷酸酶Two protein release factors (RFs) showing codon specificity, RF-1 (UAG, UAA) and RF-2 (UAA, UGA),... G Grentzmann,D Brechemierbaey,V Heurguéhamard,... - 《Journal of Biological Chemistry》 被引量: 66发表: 1995年 LepA调节蛋白翻译的机...
The N-terminal region of the protein, consisting of GTPase and 尾-barrel domains, is common to all translational GTPases. BipA domains III and V have structural counterparts in EF-G and LepA. However, the C-terminal domain (CTD) of the protein is unique to the BipA family. To ...
Moreover, ribosome binding assays indicated that EF-G, RF3, and IF2 require L7/L12 for stable binding in the GTP state, and LepA retained > 50% binding. Lastly, an EF-GG truncation mutant possessed ribosome-dependent GTPase activity, which was insensitive to L7/L12. Our results indicate ...