To investigate this energy-demanding process, we developed second-generation ratiometric biosensors for pyridine nucleotides which are pH insensitive between pH 7.0 to pH 8.5. By monitoring dynamic changes in ATP and NADPH concentrations and the NADH/NAD+ ratio at the subcellular level in Arabidopsis ...
Tube formation assay was performed using 96-well plates coated with Matrigel (Corning Inc., Tewksbury, MA, USA). Matrigel was thawed at 4 °C overnight. The solution was added into the wells of a 96-well plate (50 μl per well), and incubated at 37 °C for 1 h to allow...
First, the growth on Mycobacteria agar plates was quantified during four days after electroporation. The growth was compared to a strain harbouring only the vector pMV306. As it is shown in Figure6A to 6D, heterologous expression ofmspA,porM1andporM2led to enhanced growth of complemented strain...
Afterwards, 0.1 ml of the suspensions were aliquoted onto three replicate plates per dilution of agar-nutrient (Acumedia) media for bacteria (pH 7.0) and Martin agar media for fungi (pH 5.0). Cultures were kept at 24 °C to isolate mesophilic microorganisms and at 45 °C for thermophilic ...
Previously isolated and characterized fungal plant pathogen Fusarium oxysporum was grown on PDA plates at 25 °C for 10 days. Fungal disks were obtained using the back side of sterile 200 μL micropipette tips. To test bacteria, disks were placed in the center of new PDA plates, and the ...
The aim of this work was to investigate the most promising natural antimicrobials effective for the growth suppression of Xanthomonas spp. bacteria. The research objects were Xanthomonas spp. strains isolated from tubers and stem of plants growing in Lit
Afterwards the supernatants were transferred to 96 well plates and the absorbance was measured at 580 nm in a microplate reader (FLUOstar, BMG LabTech, Offenburg, Germany). A standard curve was prepared by measuring the absorbance for a series of trypan blue solutions at different concentrations....
C2C12 cells were obtained from The American Type Culture Collection (ATCC, Manassas, USA), and cells were cultured in 6-well plates containing 3 mL of the medium at a density of 3 × 105cells per well under 5% CO2 and 37 °C. The growth medium comprised 10% (v/v) fetal bovine...
The upper- tier plates are situated very close to the overhead lights and received about 180 μmol m-2s-1. The consequence of this close proximity was that there was very little in the way of a “vertical” gradient of light stimulus established in the upper- tier plates, and the ...
The upper- tier plates are situated very close to the overhead lights and received about 180 μmol m-2s-1. The consequence of this close proximity was that there was very little in the way of a “vertical” gradient of light stimulus established in the upper- tier plates, and the ...