green fluorescent proteinA new plasmid, pJ22, was produced by introducing the enhanced green fluorescent protein (GFP) coding sequence into the pET28 plasmid while retaining much of the multiple cloning site. This new plasmid was then used to produce a chimeric fusion protein containing the DNA-...
The detection results of stereo-fluorescence microscope show that green-fluorescent protein (GFP) gene is a kind of ideal marker gene. 立体荧光显微镜检测结果表明:绿色荧光蛋白(GFP)基因是一种理想的标记基因。 www.ebiotrade.com 10. We constructed a recombinant plasmid of HSP70 with enhanced green fl...
Fig. 3.Fluorescent protein expression by retinal neurons. (A) Transient expression of YFP by a E13 chick retinal ganglion cell obtained after ballistic delivery of plasmid to a retinal wholemount. (B) Orthogonal rotation of the confocal image stack encompassing the arbor of the cell in (A), ...
Plasmid construction The DNA fragment of green fluorescent protein (GFP), used in a green Ca2+indicator G-GECO21, was synthesised by Integrated DNA Technologies (Coralville, IA, USA). This was engineered to containKpnI andBglII restriction enzyme sites between N145 and S146 and then cloned in...
Construction of Plasmid with Green Fluorescent Protein Gene for Analysis of Yeasts in Yeast Wastewater Treatment用于废水处理系统中目标酵母动态解析的绿色荧光蛋白基因质粒构建Construction of Plasmid with Green Fluorescent Protein Gene for Analysis of Yeasts in Yeast Wastewater Treatment用于废水处理系统中目标酵母...
Since its first use as a reporter gene in 1994, green fluorescent protein (GFP) has served as the researcher′s agent: slipping, virtually undetected,
The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene. 通过转化绿色荧光蛋白基因质粒,对阿特拉津降解基因工程菌进行标记。 2. Objective To establish indirect ELISA for quantitative detection of green fluorescent prote...
DESCRIPTION: The antigenomic pHMPV83 plasmid was modified by the promoter-proximal insertion of a transcription cassette containing the ORF for enhanced green fluorescent protein (GFP) (Clonetech). The GFP ORF was flanked by the 16-nt putative N gene-start (GS) sequence and a 14-nt putative ...
KeywordsgreenfIuorescentprotein;vector;pIasmidpUCl8 AbstractAim:Toconstructascreeningvector.Methods:PoIymerasechainreaction(PCR),aIkaIineIysisminiprepof pIasmidDNA,digestionofDNAwithrestrictionendonucIeases,isoIationandpurificationofIargeDNAfragmentsusingIowmeIting temperatureagarosegeIs,IigationofGFPgeneandpUCl8tr...
以便捷的单管形式包含了基于 SYBR™ Green 染料的 –PCR 扩增和检测所需的一切成分。Applied Biosystems™ SYBR™Green了解更多信息 Have Questions? 更改视图 货号数量 43349731 x 50 mL 43444631 x 1 mL 43091551 x 5 mL 43643442 x 5 mL 43643465 x 5 mL ...