We have constructed a library in Escherichia coli of mutant gfp genes (encoding green fluorescent protein, GFP) expressed from a tightly regulated inducible promoter. We introduced random amino acid (aa) substi
Just such a molecule, green fluorescent protein (GFP), has recently appeared on the scene. GFP comes from the jellyfish Aequorea victoria and is naturally fluorescent; when excited by light of one wavelength, it emits light of another wavelength. In fact, many jellyfish emit green light when...
The green fluorescent protein (Gfp) as a tool for the analysis of gene expression in mammalian cellsSubramanian, SSrienc, FSubramanian et al., “The Green Fluorescent Protein (Gfp) as a Tool for the Analysis of Gene Expression in Mammalian Cells,” in Cell & Tissue Culture: Laboratory ...
The green fluorescent protein. Annu. Rev. Biochem. 67, 509–544 (1998) Article CAS Google Scholar Download references Author information Authors and Affiliations Senior Editor, Nature Cell Biology, Sowmya Swaminathan Rights and permissions Reprints and permissions About this article Cite this article...
A. Itcauses the mutation of proP gene B. Inducing the transcription of proP gene C. Construction of gene library containing green fluorescent protein D. Green fluorescent protein as a reporter gene to report the promoter activity of proP gene E. The first step of cloning proP gene into other...
Separately, two different methods in bacteria link target binding by a catalytically dead nuclease to green fluorescent protein fluorescence or growth that both enrich for recognized PAM sequences25,46. Finally, cell-free transcription–translation systems (TXTL) offer a more rapid and scalable means...
The Discovery of Aequorin and Green Fluorescent Protein (GFP).This article focuses on the discovery of aequorin and green fluorescent protein (GFP). Aequorin and GFP was discovered in 1961 from the same species of jellyfish. The target was a luminescent substance, aequorin, and GFP was isolated...
and mutation and evolution of such proteins has led to the creation of variants including the “fruit” FPs (e.g., tdTomato, mStrawberry, mCherry, mPlum, and mRaspberry), specialized FPs such as the fluorescent timer protei...
Green fluorescent protein (GFP) and red fluorescent protein (RFP) expression in 4X cloned aggregates using the empty zona method.ChiHun ParkYoungHee JeongDongKyung LeeJae Yeon HwangKyungJun UhSuCheong YeomCurie AhnChangKyu Lee
The rapid cessation of transcription from Para after the shift to glucose was evident in control experiments with a strain that expressed the green fluorescent protein (GFP) from the arabinose promoter (Additional file 3 - Figure S1) YgjD depletion leads to a change in cell size homeostasis ...