A set of 28 marker genes was selected based on previous studies on aquatic microbial communities [43,44,45] and screened using Eggnog-Mapper annotations to further investigate processes related to carbon, nitrogen, phosphorus, sulfur and iron metabolisms. KO identifiers, corresponding processes, enzy...
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Then, the current density is analyzed by the Pinchpoint Mapper tool in the Linkage Mapper toolbox. According to the natural breaking point method, the current density is divided into five grades, and the area with the largest grade current density is selected as pinch points. After many ...