Gram staining in Microbiology for second-year MBBS students: Comparative study of student scores in Objective Structured versus Traditional Practical Examinationdoi:10.9790/7388-0704010104Milind R. UbaleRajani P. ToreSundaram KartikeyanAniruddha A. Malgaonkar...
Gram stain is probably one of the most commonly used staining procedures used in the field of microbiology. It is one of the differential stains that are used to characterize bacteria in one of two groups: either gram positive bacteria or gram negative bacteria. ...
Introduction to the microbiology of meat and meat products Gerhard Feiner, in Meat Products Handbook, 2006 38.8 Gram-positive and Gram-negative bacteria For classification purposes, bacteria can be stained (coloured) and Gram staining, named after the Danish microbiologist Hans Christian Gram (1853–...
Full size image Membrane and cytoplasmic co-staining suggest that defects in early PG assembly cause aberrant morphology and cell lysis To better understand how periplasmic filament assembly affects cell viability, we investigated the effects of the absence of FlhE on OM and IM integrity using simult...
(CITA-IVIA), Castellón, Spain; 4Skirball Institute Program in Molecular Pathogenesis and Departments of Microbiology and Medicine, Skirball Institute, New York University Medical Center, New York, NY, USA and 5Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National ...
Our new in situ AFM imaging method avoided drying or staining artefacts; AFM requires no staining and image features were unaffected by whether the sample was dried during preparation (Supplementary Fig. S1c,d). Figure 2: Disruption of peptidoglycan architecture visualized by AFM. (a) Targets ...
3C). The possible explanation of the good therapeutic effect of AOA-2 could be the fact that AOA-2 would affect the interaction of bacteria with host cells which prevents their evasion from the immune host defence. These results are consistent with previous observations that absence of OmpA in...
In the process of Gram staining, a slide containing a heat-fixed smear of bacterial cells is treated with crystal-violet stain. The slide is then flushed with aniodinesolution, followed by an organicsolvent, such asalcoholoracetone. In the final step, a counterstain, such as safranin, is ad...
Gram staining is carried out by first heat-fixing bacterial cells to a slide. The slide is then treated with crystal-violet stain, followed by washing with an iodine solution. In the final steps the slide is washed with an organic solvent, such as alcohol or acetone, and a counterstain, ...
Ten-fold dilutions of the homogenized spleen, lungs and blood were plated onto sheep blood agar (Becton Dickinson Microbiology Systems, USA) for quantitative cultures. If no growth was observed after plating the whole residue of the homogenized tissue and blood, a logarithm value corresponding to ...