In aqueous solutions, crystal violet dissociates into CV+ and Cl – ions that penetrate through Gram-positive and Gram-negative cell walls. The CV+ interacts with negatively charged components of bacterial cells, staining the cells purple. When added, iodine (I- or I3-) interacts with CV+ to...
In basic Laboratories, the simplest endospore staining technique is the Schaeffer-Fulton technique because of its easy and it is rapid to identify the bacteria. It applies the use ofMalachite green dye(alkaline solution with a pH of 11-11.2 and its a water-soluble dye) along with the use of...
Department of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany 5 Mycology Laboratory, National Institute for Amazonian Research (INPA), Manaus 69067-375, Brazil * Authors to whom correspondence should be addressed. J. Fungi 2022, 8(1), 13; https:/...