Patane G, Anello M, Piro S, Vigneri R, Purrello F, Rabuazzo AM 2002 Role of ATP production and uncoupling protein-2 in the insulin secretory defect induced by chronic exposure to high glucose or free fatty acids and effects of peroxisome proliferator-activated receptor-gamma inhibition. ...
In total 10,000 cells were used to produce each RNA-seq library, with two replicates for each genotype and experimental condition. RNA was extracted into a 30-µL volume using the RNeasy Mini Kit (74104, Qiagen) and RNase-Free DNase Set (79254, Qiagen), following the manufacturer’s in...
Furthermore, nanoparticles are known to induce cytotoxicity by inducing apoptosis, depletion of ATP and necrosis [76]. This may also be increasing their cytotoxicity at day 1 and day 3. By day 5, there was no significant difference in cytotoxicity between the high dose of the NPs and the ...