For DNA digestion of single and double-stranded DNA, 1 μg RNA sample was incubated at room temperature for 10 min with 1 μl 10× DNase I Reaction Buffer, 1 μl DNase I Amp Grade 1 U/μl, and DEPC-treated water to final volume of 10 μl. DNase I was inactivated by incubation ...
HPLC-grade methanol and formic acid were purchased from Tedia Company (Fairfield, OH, USA). Ultrapure water (18.2 MΩ) was obtained from an ELGA–pure lab Ultra system (High Wycombe, UK). M6G-d3 was labeled by Biomag System Company (Changshu, Jiangsu, China). LEICA inverted ...
Sequencing grade modified trypsin (Promega) was prepared to 0.01 μg/μl in 50 mm NH4HCO3, and ∼50 μl of this was added to each gel band so that the gel was completely submerged. Bands were then incubated at 37 °C overnight. Peptides were extracted by water bath sonication in a...
(v/v) for 5 h followed by pure medium-grade acrylic LR White resin (Agar Scientific,http://www.agarscientific.com) for 2 days, taking care to replace the resin every day. Samples were finally placed in the center of capsules and placed in a heat chamber for 24 h (67 °C). ...
Application Abbreviations 65 Application Abbreviations Vacn Var Ver Vol WAO WCB WIGI wrk XFER XLAT XSLT YTD ZVW Appendix A Vacation Variable Version Volume Disability Insurance Act (Dutch: Wet op de arbeidsongeschiktheidsverzekering) Workers' Compensation Board Within Grade Increases Worker Transfer ...
Six of these events were categorized as grade 2, while the other events were graded as grade 1. The reference product and the test product both had TEAEs counts of 1413 each.Concerning safety and bioequivalence, both suspension formulations of Oseltamivir phosphate are comparable.The two ...
Application Abbreviations 65 Application Abbreviations Vacn Var Ver Vol WAO WCB WIGI wrk XFER XLAT XSLT YTD ZVW Appendix A Vacation Variable Version Volume Disability Insurance Act (Dutch: Wet op de arbeidsongeschiktheidsverzekering) Workers' Compensation Board Within Grade Increases Worker Transfer ...