In the case of the M5R, β-arrestin1 recruitment was completely abolished in ΔQ-GRK. Still, a major difference in GRK-selectivity of the two receptors was found using this approach: the individual overexpression of GRK2, 3, 5 and 6 significantly increased β-arrestin recruitment to the PTH...
5). Consequently, since binding is depleted in the presence of the W36K mutant and is not impeded in the case of the mutated forms of the CSH3 and SH2 domains, our data suggest that AGO2 is recruited to GRB2 NSH3 but not to the CSH3 nor SH2 domains. To validate this observation...
TrypLE™ Express Enzyme (12604021, Thermo Scientific, Massachusetts, USA) was used to digest and resuspended cancer cells for organoid passages. Co-immunoprecipitation (Co-IP) assay After incubation with 50 nM dexamethasone for 48 h, cancer cells (2 × 106) were washed with PBS, and...
Web Services Authentication with .NET – HTTP Digest Sample I have posted anarticle and sampleof using HTTP Digest authentication with web services, without using the built-in IIS Digest implementation and Active Directory. Like myprevious authentication sample, this one is all managed code, and wi...
Notably, GRK5 in the absence of CaM can still sample an open conformation, albeit with average interdomain distances reduced to ∼17 Å (Figure 3C, orange traces). Although GRK5 is not bound to CaM, the N terminus still maintains an α-helical fold (Figure S4D, orange RMSD αN ...
In that case, maize pollen was germinated in optimal conditions and the effects of emmer extracts were evaluated considering only the extracts of free phenolic compounds (i.e., those obtained by extraction with methanol). The present work follows up the work by Del Pino et al. [16], by ...
SAMPLE-TO-SNP Thermo Fisher Scientific Inc. Sanicult Thermo Fisher Scientific Inc. SAPPHIRE Thermo Fisher Scientific Inc. SAPPHIRE-II Thermo Fisher Scientific Inc. Sartopore Satorius Savant Thermo Fisher Scientific Inc. SAV-IT Thermo Fisher Scientific Inc. SC-CSRS Thermo Fisher Scientific Inc. Scint...
Just in case you come into contact with hepatitis B – your body will then say “I know you” and will stop the virus from hurting your liver. So if a virus comes at you like the snowstorm, your body says “I know you” and stops the virus from hurting you. * We talked about ...
(); MessageDigest md = MessageDigest.getInstance("SHA-256"); md.update(cert); hashes.add(toHexString(md.digest())); } Collections.sort(hashes); StringBuilder hash = new StringBuilder(); for (int i = 0; i < hashes.size(); i++) { hash.append(hashes.get(i)); } return hash....
Email Digest Frequency emailDigestFrequency string Workspace ID workspaceId integer Permissions permissions array of Disable History disableHistory boolean ssoIntegrationId ssoIntegrationId anyVariableValue The value of the variable. ssoAuthPartnerId ssoAuthPartnerId anyVariableValue The value of the vari...