In the case of the M5R, β-arrestin1 recruitment was completely abolished in ΔQ-GRK. Still, a major difference in GRK-selectivity of the two receptors was found using this approach: the individual overexpression of GRK2, 3, 5 and 6 significantly increased β-arrestin recruitment to the PTH...
Genes of interest were amplified using polymerase chain reaction (PCR) and, following dedicated restriction enzyme digests, DNA ligations were established in 10 μl volumes with a 3:1 ratio of insert:vector DNA using T4 DNA ligase (NEB #M0202) and DNA ligation buffer (NEB #M2200). The...
TrypLE™ Express Enzyme (12604021, Thermo Scientific, Massachusetts, USA) was used to digest and resuspended cancer cells for organoid passages. Co-immunoprecipitation (Co-IP) assay After incubation with 50 nM dexamethasone for 48 h, cancer cells (2 × 106) were washed with PBS, and...
()).hexdigest() return params + @staticmethod + def _validate_w_webid(w_webid): + if not w_webid: + return False + decoded = jwt_decode_hs256(w_webid) + created_at, ttl = decoded.get('created_at'), decoded.get('ttl') + if not isinstance(created_at, int) or not ...
Originality/value ‐ The briefing saves busy executives and researchers hours of reading time by selecting only the very best, most pertinent information and presenting it in a condensed and easy-to digest format.doi:10.1108/02580541211212763None...
digestAlgorithm True string Select the message digest algorithm Output Type cryptoOutputDataType string Set the data format for the hash code generated. Case case string Set the case applied to the computed value. Encoding Type encoding string Select the encoding type. Returns Body DtoResponse...
License to copy and use this software is granted provided that it is identified as the "RSA Data Security, Inc. MD5 Message- Digest Algorithm" in all material mentioning or referencing this software or this function. License is also granted to make and use derivative works provided that such ...
FormatSelection FormattingToolbar FormDigest FormInstance FormPostBodyParameterNode FormPostBodyStringNode FormTag Forwards Forwardslash FourColumns FourRows FourthOfFourColumns FourthOfFourRows Fragment FrameBorder FrameContainer FrameSelect FrameSet Framework FrameworkDesignStudio FrameworkError FrameworkPrivate Framew...
(); MessageDigest md = MessageDigest.getInstance("SHA-256"); md.update(cert); hashes.add(toHexString(md.digest())); } Collections.sort(hashes); StringBuilder hash = new StringBuilder(); for (int i = 0; i < hashes.size(); i++) { hash.append(hashes.get(i)); } return hash....
# the file digest algorithms to use on the scanned file (options: "md5", "sha1", "sha224", "sha256", "sha384", "sha512") digests: ["sha256"] # options when pulling directly from a registry via the "registry:" or "containerd:" scheme registry: # skip TLS verification when comm...