The protein density in biological membranes can be extraordinarily high, but the impact of molecular crowding on the diffusion of membrane proteins has not been studied systematically in a natural system. The diversity of the membrane proteome of most cells may preclude systematic studies. African ...
There is some discussion as to whether glycosyl-phosphatidylinositol(GPI)-anchored proteins occur in microdomains in the cell membrane. These putative microdomains have been implicated in processes such as sorting in polarized cells and signal transduction. Complexes enriched in GPI-anchored proteins, ...
It was envisaged that if glycosylphosphatidylinositol (GPI)-specific PLC was activated in the membrane of psoriatic epidermal cells, it would render these cells devoid of those proteins which are anchored to the cell membrane through their GPI moiety. In order to test this possibility, four GPI ...
Dropping anchor: Glycosylphosphatidylinositol (GPI) membrane-anchored proteins noncovalently associate with the plasma membrane and can have an impact on oncogenesis and some infectious diseases. The GPI anchor biosynthetic machinery and GPI-T, the transamidase that attaches them to proteins, are ...
Lipid anchored proteinsPost-translational modificationProtein sortingPredictionNeural networksGPI-anchors constitute a very important post-translational modification, linking many proteins to the outer face of the plasma membrane in eukaryotic cells. Since experimental validation of GPI-anchoring signals is slow...
letoftheplasmamembrane,whileproteinsarenotdi- rectlyinsertedintothemembrane(Fig.1A).GPIanchor istheonlyformofposttranslationalmodificationby glycolipid. Morethan150differenthumanproteinsareGPIan- chored(UniProt)(9).FunctionsoftheseGPI-anchored AbstractGlycosylphosphatidylinositols(GPIs)actasmem- braneanchors...
It was found that a deficiency of GPI-anchored membrane proteins in PNH cells is due to the faulty synthesis of the GPI-anchor which may occur during early synthesis. Moreover, it is suggested that an abnormality of the PIG-A (phosphatidylinositol glycan-class A) gene, which is related to...
The plasma membrane domain to which the GPI-anchored protein localizes also depends on the cell type; for example, in Madin-Darby canine kidney (MDCK) cells, GPI-anchored proteins are present mainly on the apical surface. On the contrary, in Fisher rat thyroid cells, they are sorted to ...
Journal of Membrane BiologyJ. Wang, W. Gunning, K.M. Kelley, M. Ratnam, Evidence for segregation of heterologous GPI-anchored proteins into separate lipid rafts within the plasma membrane, J. Membr. Biol. 189 (2002) 35-43.Wang J, Gunning W, Kelley KM, Ratnam M. Evidence for ...
While the contribution of the membrane-embedded domain to diffusion is well established, the potential impact of the extra-membrane protein part has been largely neglected. Here, we show that the molecular length influences the diffusion coefficient of GPI-anchored proteins: smaller proteins diffuse ...