Cell Meter Beta-Arrestin Translocation GPCR Signaling Kit 一、化学性质与结构 Cell Meter Beta-Arrestin蛋白易位GPCR信号检测试剂盒是一套基于β-Arrestin蛋白易位机制，结合荧光标记检测的细胞信号转导分析系统。其核心组成包括：荧光标记的β-Arrestin融合蛋白表达质粒 GPCR激动剂/拮抗剂处理体系 荧光成像试剂和缓冲液 ...
向小瓶β-arrestin-GFPDNA(组分A)中加入10 μL ddH2O,充分混合至终浓度为1 μg / μL。 操作步骤 1.将3 μg DNA(例如1.5 μg Beta-arrestin-GFP DNA储备液和1.5 μg您准备的GPCR DNA)与200 μL无血清培养基混合。 2.向混合物中加入9 μL Transfectamine 5000(组分B)。 3.充分混合并在室温下孵...
G-protein coupled receptors (GPCRs) initiate signaling cascades via G-proteins and beta-arrestins (βarr). βarr-dependent actions begin with recruitment of βarr to the phosphorylated receptor tail and are followed by the engagement with the receptor core. βarrs are known to act as adaptor...
include the HTRF GTP Gi binding kit, the industry’s first TR-FRET based assay for GTP binding; the B-arr2 recruitment kit; and HTRF total kits for B-Arrestin 1, B-Arrestin 2 and AP2. These assays will help scientists continue to better understand the important role GPCRs...
品名:PathHunter eXpress MC4R U2OS Beta-Arrestin GPCR Assay 规格:1,000 dp (10 x 96-well) 货号:93-0211E3CP0L 一、我们关于PathHunter eXpress MC4R U2OS Beta-Arrestin GPCR Assay蛋白、试剂盒、补体的声明 我们致力于成为科研试剂搜索、采购一体化解决方案提供商!
β-arrestin-GFPDNA储备溶液向小瓶β-arrestin-GFPDNA(组分A)中加入10 µL ddH2O,充分混合至终浓度为1 µg / µL。 操作步骤 1.将3 µg DNA(例如1.5 µg Beta-arrestin-GFP DNA储备液和1.5 µg您准备的GPCR DNA)与200 µL无血清培养基混合。2.向混合物中加入9 µL Transfectamine 5000...
The structure provides evidence for the ability of a GPCR to activate G protein even while being bound to and internalized by βarr. It also reveals that the binding of G protein and βarr to the same GPCR is not mutually exclusive, and raises a number of future questions to be answered...
β-arrestins bind G protein-coupled receptors to terminate G protein signaling and to facilitate other downstream signaling pathways. Using single-molecule fluorescence resonance energy transfer imaging, we show that β-arrestin is strongly autoinhibited in its basal state. Its engagement with a ...
GPCR endosomal signalingPAC1 receptorβ-arrestinERK activationStress-related disordersJournal of Molecular Neuroscience - G protein-coupled receptors (GPCRs) are currently appreciated to be routed to diverse cellular platforms to generate both G protein-dependent and -independent......
β -Arrestins are associated with numerous aspects of GPCR signaling and regulation, and accordingly influence diverse physiological and pathophysiological processes. The objective of this study was to determine whether b -arrestins a re involved in GPCR-mediated beta-amyloid peptide generation in vitro...