6. Buck, A.K., et al., CXCR4-targeted theranostics in oncology. Eur J Nucl Med Mol Imaging, 2022. 49(12): p. 4133-4144. 7. Kularatne, S.A., et al., A CXCR4-targeted site-specific antibody-drug conjugate. Angew Chem Int Ed Engl, 2014. 53(44): p. 11863-7. 8. Wang, R...
[8] Li T, Vandesquille M, Koukouli F, et al. Camelid single-domain antibodies: A versatile tool for in vivo imaging of extracellular and intracellular brain targets[J]. Journal of Controlled Release, 2016,243:1-10. [9] Che T, English J, Krumm B E, et al. Nanobody-enabled monitori...
2024年2月2日,北大-清华生命科学联合中心自振滔和饶毅发表一篇论文,主要三点:首先,GPR39有新的配体:胆酸;其次,发现胆酸作用的新信号途径;第三,发现胆酸可以通过GPR39而引起急性胰腺炎。 GPR39是七重跨膜的G蛋白偶联受体(GPCRs)之一。...
[4] Timo W.M. De Groof, Vladimir Bobkov, Raimond Heukers, Martine J. Smit,Nanobodies: New avenues for imaging, stabilizing and modulating GPCRs,Molecular and Cellular Endocrinology,Volume 484,2019,Pages 15-24. [5]Rasmussen, S. G. et al. Structure of a nanobody-stabilized active state ...
2022年9月12日,中国科学院深圳先进技术研究院生物医学与健康工程研究所储军研究组在Nature Communications上发表题为“A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging”的最新研究成果。 研究团队...
利用奥德赛红外线成像系统(Odyssey Infrared ImagingSystem, L1-cor Biosciences)扫描斑点印迹并使之成像。因为两种不同的通道(分别为800nm对700nm)用于成像,因此通过Ml从检测β 2AR的印迹分别处理检测纳米抗体的印迹;因此不能直接比较并定量印迹(即纳米抗体的结合与Ml结合的比较本质上是定性的)。(b)代表性斑点印迹...
6.Buck, A.K., et al., Buck, A.K., et al.,CXCR4-targeted theranostics in oncology.Eur J Nucl Med Mol Imaging, 2022.49(12): p. 4133-4144. 7.Kularatne, S.A., et al., Kularatne, S.A., et al.,A CXCR4-targeted site-specific antibody-drug conjugate.Angew Chem Int Ed Engl,...
of labeling methods and the improvement of measuring and analyzing instrumentation, have made fluorescence techniques to an indispensable tool for GPCR imaging. The illumination of their complex life cycle, which includes receptor biosynthesis, membrane targeting, ligand binding, signaling, internalization, ...
in vivo imagingneutrophilsT cellsG-protein-coupled receptor (GPCR) signaling is essential for the spatiotemporal control of leukocyte dynamics during immune responses. For efficient navigation through mammalian tissues, most leukocyte types express more than one GPCR on their surface and sense a wide ...
5. Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44. 6. Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bil...