The lysosomal limiting membrane contains a high concentration of integral membrane proteins including the lysosome-associated membrane protein 1 (LAMP-1), LAMP-2, and lysosomal integral membrane proteins 1 and 2 (LIMP-1/CD63 and LIMP-2) ( Eskelinen et al. 2003 ). Mature lysosomes may ...
Lysosome biogenesis requires Rab9 function and receptor recycling from endosomes to the trans-Golgi network. Newly synthesized lysosomal enzymes bind to mannose 6-phosphate receptors (MPRs) in the TGN, and are carried to prelysosomes, where they are released. MPRs... MA Riederer,T Soldati,AD ...
The biosynthesis, glycosylation, movement through the Golgi system, transport to lysosomes, and turnover of three lysosomal integral membrane proteins (LIMPSs) have been studied in normal rat kidney cells using specific anti-LIMP monoclonal antibodies. Immunoelectron microscopy studies revealed the ...
Blocking lysosomal transport of APP reduces Aβ production by more than a third. Conclusion These data suggests that AP-3 mediates rapid delivery of APP to lysosomes, and that the lysosome is a likely site of Aβ production.Introduction AD is the leading cause of dementia in adults [1]. A...
Blocking lysosomal transport of APP reduces Aβ production by more than a third. Conclusion: These data suggests that AP-3 mediates rapid delivery of APP to lysosomes, and that the lysosome is a likely site of Aβ production. 展开 关键词: Lysosome Confocal microscopy Trafficking Gamma-secretase...
Endoplasmic reticulum,Lysosome The best understood of the processing reactions carried out by the Golgi apparatus is the remodeling of oligosaccharides (chains of six-carbon sugars) that are attached to glycoproteins. During insertion of a newly synthesized protein into the endoplasmic reticulum, one or...
Lysosome PM Bafilomycin A1: –– + –– + –– + 75 50 37 37 75 GLUT1 GAPDH ← 50 SNX27 NS 1.0 ** * 0.8 0.6 0.4 0.2 0.0 siCTL siSNX27 3xFLAG-SNX27-r Figure 3 | The interaction of SNX27 with FAM21 is required for proper recycling of its cargo to the plasma membrane (PM...
To directly examine YIPF3 and YIPF4 as autophagic substrates, we fused the fluorescent Keima protein to YIPF3 and YIPF4 (Extended Data Fig.7b). Keima undergoes a change in chromophore resting state upon trafficking to the acidic lysosome compartment (pH ≈ 4.5), allowing flux measurements ...
The CD-MPR is one of two transmembrane proteins that bind mannose-6-phosphate (M6P) tags on acid hydrolase precursors in the Golgi apparatus that are destined for transport to the lysosome. Els receptors de la mannosa o CD206 són dues proteïnes transmembrana que uneixen la manosa 6-...
such as viruses and plant or bacterial toxins, can also hijack this pathway to invade cells while avoiding lysosomal degradation. As a major cellular recycling pathway, the endosome-to-Golgi trafficking has been recognized for its roles in the post-Golgi secretion, biogenesis of the lysosome, main...