We found that among GM-CSF bone marrow–derived cell populations, monocyte-derived macrophages, rather than BMDCs, were responsible for inflammasome activation and IL-1β secretion. Therefore, GM-CSF bone marrow–derived cells should not be used to draw conclusions about DC-dependent inflammasome ...
Interestingly, the GM-BMM population increased but GM-BMDCs decreased in a GM-CSF dose-dependent manner. Functionally, GM-BMMs showed extremely high phagocytic abilities and produced higher IL-10 upon LPS stimulation. GM-BMDCs, however, could not phagocytose as well, but were efficient at ...
Generally, after 8-10 days culturing with GM-CSF, the suspended cells are used as DC and cells adherent to culture system are excluded in order to increase purity of BMDCs [11,12]. Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask ...
We found that when the extracellular domain of HER2/neu fused to ovalbumin (OVA-ECD(HER2)) is bound by HER2/neu-specific antibody-(IL-2) or antibody-(GM-CSF) fusion proteins, the bound antigen is more efficiently processed by murine bone-marrow-derived dendritic cells (BMDCs) and presented...
[ Abstract] 摇 Objective:To investigate the effect of IL鄄17A on the differentiation and maturation of murine bone marrow鄄derived dendritic cells( BMDCs) ... 刘腾丽摇,乔摇,赛摇,... 被引量: 0发表: 0年 自体DC疫苗对恶性黑色素瘤术后患者免疫功能的影响 目的评价恶性黑色素瘤(malignant melanoma...
Mouse bone marrow-derived dendritic cells (BMDCs) are being employed as an important model for translational research into the development of DC-based therapeutics. For such use, the localization and specialized mobility of injected BMDCs within specific immune tissues are known to define their ...
[ Abstract] 摇 Objective:To investigate the effect of IL鄄17A on the differentiation and maturation of murine bone marrow鄄derived dendritic cells( BMDCs) 郾 Methods: Murine bone marrow cells were isolated and cultured in RPMI1640 complete medium in the presence of GM鄄CSF(20 ng / ml) for ...
To detect the biological activity of GM-CSF expressed by the recombinant virus, bone marrow-derived dendritic cells (BMDCs) were isolated and co-cultured with the recombinant virus or parental virus and the surface phenotypes of BMDCs were examined by flow cytometric analysis. The cytokines secreted...