10. Anerobic Respiration Topic summary 1 concept Glycolysis Overview Video duration: 3m 2 concept Glycolysis Steps Video duration: 11m AI tutor 0 Show Answer AI tutor 0 Show Answer AI tutor 0 Show Answer Take your learning anywhere! Prep for your exams on the go with video lessons and practi...
The measured fluorescence of Cyto-pHluorin was called FMax and was background subtracted, as explained above. Cytosolic pH of the basal and Oligomycin treatments were determined using the following modified Hender- son-Hasselbalch equation: pH Basal = pKa − log[( 1+10pKa−7.4 FBasal )...
MA, USA). Briefly, after mechanical stretching, the cells were washed with PBS and then incubated with the JC-1 working solution in the dark for 10 minutes at 37 °C. After two washing using PBS, the cells were added with a culture medium and placed in a Cell Discoverer 7 (Zeiss...
Y260 phosphorylation of α1 Na/K-ATPase was detected in Src-rescued, but not in parental, SYF cells (Fig. 2c). To address the specificity of this regulation, we also measured Y10 phosphorylation in α1 Na/K-ATPase that is mediated by insulin signaling20. Y10 phosphorylation was ...