We provide in vivo evidence that NMNAT2 in glutamatergic neurons is required for axonal survival. Using in vivo and in vitro studies, we demonstrate that NMNAT2 maintains the NAD-redox potential to provide “on-board” ATP via glycolysis to vesicular cargos in distal axons. Exogenous NAD+ suppl...
MA, USA). Briefly, after mechanical stretching, the cells were washed with PBS and then incubated with the JC-1 working solution in the dark for 10 minutes at 37 °C. After two washing using PBS, the cells were added with a culture medium and placed in a Cell Discoverer 7 (Zeiss...