Glucan 1, 3-alpha-glucosidasedoi:10.1007/978-3-642-84437-9_73Schomburg, DietmarGBF - Gesellschaft für Biotechnologische Forschung mbHSalzmann, MargitGBF - Gesellschaft für Biotechnologische Forschung mbHSpringer Berlin Heidelberg
A very good example of beta-glucan catabolic enzymes is the Family 3 ofglycosyl hydrolases(GH3), which consists of nearly 44 beta-glucosidases andhexosaminidasesfrom bacteria, molds, and yeasts. Most of the fungal beta-glucosidases studied belong to GH3. Structural data on representatives of GH3...
3057α-Glucosidase rapidly hydrolyzes these glycosides. Maltooligosaccharide derivatives incorporating genipin [methyl (1R,2R,6S)-2-hydroxy-9-(hydroxymethyl)-3-oxabicyclo[4.3.0]nona-4,8-diene-5-carboxylate, 2], used for analysis of alpha amylases, have been prepared from geniposide (3) and a ...
Crystallization experiments were conducted at 25 °C using the sitting-drop vapor-diffusion method. 10 mg/mL protein was mixed with reservoir solution in a 1:1 ratio. The crystals were obtained using the “1–28” condition of the Morpheus II screen kit (Molecular Dimensions; 0.1 M ...
Only two of the newly identified enzymes, a putative α-glucosidase (AgdB) and an α-amylase (AmyC), were predicted to play a role in starch degradation. The expression of the majority of the genes identified was not induced by maltose as carbon source, and not dependent on the presence ...
overcomes these limitations. In this assay, highly purified endo-1,3(4)-β-glucanase (lichenase) and β-glucosidase are employed. The glucan is depolymerized by lichenase to oligosaccharides, these oligosaccharides are quantitatively hydrolyzed by β-glucosidase to glucose, and this is specifically ...
Briefly, β-glucan was hydrolyzed by lichenase into oligosaccharides, which were converted into glucose by β-glucosidase. The amount of glucose released was measured by UV absorbance with glucose oxidase/peroxidase. The β-glucan content was calculated based on the amount of released glucose. ...
To determine the minimum amount of oat β-glucan (OBG) required to reduce glycaemic responses (MinDose), we conducted a systematic review and meta-regression analysis of acute, crossover, single-meal feeding trials that examined the effects of adding OBG
Briefly, β-glucan was hydrolyzed by lichenase into oligosaccharides, which were converted into glucose by β-glucosidase. The amount of glucose released was measured by UV absorbance with glucose oxidase/peroxidase. The β-glucan content was calculated based on the amount of released glucose. ...
any remaining glucan fragments were quantitatively hydrolyzed to glucose using a mixture ofexo-1,3-β-glucanase and β-glucosidase which gives a measurement of total glucan. The alpha glucan and sucrose content of the sample is determined by hydrolyzing specifically tod-glucose andd-fructose. Glucose...