Glucan 1, 3-alpha-glucosidasedoi:10.1007/978-3-642-84437-9_73Schomburg, DietmarGBF - Gesellschaft für Biotechnologische Forschung mbHSalzmann, MargitGBF - Gesellschaft für Biotechnologische Forschung mbHSpringer Berlin Heidelberg
A very good example of beta-glucan catabolic enzymes is the Family 3 ofglycosyl hydrolases(GH3), which consists of nearly 44 beta-glucosidases andhexosaminidasesfrom bacteria, molds, and yeasts. Most of the fungal beta-glucosidases studied belong to GH3. Structural data on representatives of GH3...
3057α-Glucosidase rapidly hydrolyzes these glycosides. Maltooligosaccharide derivatives incorporating genipin [methyl (1R,2R,6S)-2-hydroxy-9-(hydroxymethyl)-3-oxabicyclo[4.3.0]nona-4,8-diene-5-carboxylate, 2], used for analysis of alpha amylases, have been prepared from geniposide (3) and a ...
α-glucans, mainly referring to starch and glycogen, are the predominant carbohydrates accumulated for energy storage in nature1; their utilization, for instance, as main human diet constituents and food-industry raw materials, is increasing globally because of their low cost, wide distribution, and ...
Only two of the newly identified enzymes, a putative α-glucosidase (AgdB) and an α-amylase (AmyC), were predicted to play a role in starch degradation. The expression of the majority of the genes identified was not induced by maltose as carbon source, and not dependent on the presence ...
Briefly, β-glucan was hydrolyzed by lichenase into oligosaccharides, which were converted into glucose by β-glucosidase. The amount of glucose released was measured by UV absorbance with glucose oxidase/peroxidase. The β-glucan content was calculated based on the amount of released glucose. ...
To determine the minimum amount of oat β-glucan (OBG) required to reduce glycaemic responses (MinDose), we conducted a systematic review and meta-regression analysis of acute, crossover, single-meal feeding trials that examined the effects of adding OBG
Effects of 8-wk alpha-glucosidase inhibition on metabolic control, C-peptide secretion, hepatic glucose output, and peripheral insulin sensitivity in poorly controlled type II diabetic patients. Diabetes Care 12, 537–543. Article CAS Google Scholar Scott CL (2003). Diagnosis, prevention, and ...
overcomes these limitations. In this assay, highly purified endo-1,3(4)-β-glucanase (lichenase) and β-glucosidase are employed. The glucan is depolymerized by lichenase to oligosaccharides, these oligosaccharides are quantitatively hydrolyzed by β-glucosidase to glucose, and this is specifically ...
any remaining glucan fragments were quantitatively hydrolyzed to glucose using a mixture ofexo-1,3-β-glucanase and β-glucosidase which gives a measurement of total glucan. The alpha glucan and sucrose content of the sample is determined by hydrolyzing specifically tod-glucose andd-fructose. Glucose...