glpX gene deletion does not completely abolish the FBPase activity in Δglpx: FBPase Activity was measured in nmol/min/mg protein in crude extracts, mean of two determinations, limit of detection ...
coli genome, the glpX gene forms an operon (glpFKX) with glpF (a glycerol transport facilitator) and glpK (a glycerol kinase). This operon is one of five operons of the glp regulon involved in the growth of E. coli on glycerol (9). A BLAST analysis of the E. coli genome using ...
RNA-seq HALLMARK Gene Set Enrichment Analysis (GSEA) analysis of Gsk3β KD revealed a positive enrichment of apoptosis, tumor suppressor gene, and negative enrichment of oncogenic pathway. Furthermore, combinational use of a Gsk3β inhibitor with anti-Smo or Gli1 significantly inhibited the CRPC...
glpX geneFBPasecarbon metabolism[目的]构建耻垢分枝杆菌(Mycobacterium smegmatis)glpX基因敲除株,研究其在生理代谢中的功能.[方法]利用分枝杆菌噬菌体Che9c重组系统构建耻垢分枝杆菌glpX基因敲除株;比较野生株及突变株在不同碳源培养条件下的生长差异;通过荧光实时定量PCR,比较野生株在以葡萄糖或油酸为唯一碳源培养下,...
Relevance of glp regulon gene products in mediating glycerol and sn-glycerol 3-phosphate utilization; Inhibition of the purified enzyme by adenosine diphosphate and phosphate; Enzyme activation by phosphoenolpyruvate.DonahueJanetL.BownasJenniferL.