Our Custom Protein Service offers all aspects of protein expression, production, purification, and development, from gene to protein including:: Cloning Subcloning Generation of plasmid DNA Multiprotein Complex Expressions Optimization of expression conditions Small, mini- or large-scale expression and aff...
HEK293 cells (5×104) are seeded in a 96-well plate and transiently cotransfected with the GLP-1R plasmid and the CRE-luciferase reporter plasmid. After a 48 h transfection, different concentrations of GLP-1(1–37) or GLP-1(7–37) are added, and the cells are incubated for 5 h. Th...
HEK293 cells (5×104) are seeded in a 96-well plate and transiently cotransfected with the GLP-1R plasmid and the CRE-luciferase reporter plasmid. After a 48 h transfection, different concentrations of GLP-1(1–37) or GLP-1(7–37) are added, and the cells are incubated for 5 h. Th...
Our Custom Protein Service offers all aspects of protein expression, production, purification, and development, from gene to protein including:: Cloning Subcloning Generation of plasmid DNA Multiprotein Complex Expressions Optimization of expression conditions Small, mini- or large-scale expression and aff...
4接种200ml验证正确的菌液用于质粒大提,使用试剂盒为:purelink hipure plasmid maxiprep kit,按说明书进行操作。质粒经pcr验证及酶切验证无误后,使用pvui 限制性内切酶进行线性化。最后采用乙醇沉淀法回收质粒。 [0089] 5宿主细胞为hek293,转染前一天,将细胞按照2x10^6cells/孔的密度铺6孔板,1ml/孔。使用lipofect...
Coculture assay of the GLP-1 plasmid-transfected cells with isolated rat islet cells demonstrated that GLP-1 increased insulin secretion by twofold, compared to controls during a hyperglycemic challenge. A single injection of polyethyleneimine/pbetaGLP1 complex into ZDF rats resulted in increasing ...
As a result, the GLP-1 mRNA was detected in the pβGLP1 plasmid-transfected cells (Fig. 2B). The transfected HepG2 cells expressed GLP-1 mRNA in a dose-dependent manner. This result suggests that the detected mRNA was expressed by the exogenous transferred pβGLP1 plasmid, not by the...
g, Effects of NaHS on the enzymatic activity of Gaussia Luciferase (GLUC), with GLUC secreted by HEK293T cells transfected with a GLuc-encoding plasmid (n = 4). h, Detection of H2S dissipated from NaHS-supplemented cell culture media by the lead acetate assay (n = 1). i, ...
25、所述“glp-1/gip双重激动剂(glp-1/gip dual agonist)”可以与“glp-1/gip受体双重激动剂”、“glp-1受体和gip受体双重激动剂”、“glp-1r/gipr双重激动剂”、“双重激动剂”,或“对glp-1受体和gip受体具有活性的肽”互换使用。 26、所述glp-1/gip双重激动剂可以是对glp-1受体和gip受体中的一个或更...
For replication, the plasmid may contain an origin of replication. In order to select cells trans-fected with the inventive vector, one or more antibiotic resistance gene(s) (e.g. kanamycin, ampicillin) may be provided in the vector. The vector may be a plasmid that includes bacterial ...