Immobilized Human GLP1R Protein, Fc Tag (Cat. No.GLR-H5253) at 1 μg/mL (100 μL/well) can bind GLP-1R antibody with a linear range of 0.2-8 ng/mL (QC tested). ●GGCGR高纯度:经SDS-PAGE,SEC-MALS验证 The purity of Human GCGR / Glucagon receptor Protein, Fc Tag (MALS verified...
[2] Cui X, Feng J, Wei T, et al. Pro-α-cell-derived β-cells contribute to β-cell neogenesis induced by antagonistic glucagon receptor antibody in type 2 diabetic mice. iScience, 2022, 25(7):104567. [3] Cui X, Feng J, ...
Fc融合蛋白含有的抗体Fc段,虽不能进行抗原-抗体反应,但Fc段可介导抗体依赖的细胞介导的细胞毒性(antibody-dependent cell-mediated cytotoxicity, ADCC)、补体依赖的细胞毒性(complement dependent cytotoxicity, CDC)作用等各种生物学功能,并且可以根据4种亚型介导的细胞毒差异选择融合伴侣。依靠融合伴侣的长效机制,可通过...
[9] Pyke, C., Heller, R.S., Kirk, R.K., Orskov, C., Reedtz-Runge, S., Kaastrup, P., et al. 2014. GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody. Endocrinology 155:1280-1290. [10] Baggio, L.L., ...
[left] Elisa plates were pre-coated with Flag Tag GLP1R-Nanodisc (0.2μg/per well). From above data, the EC50 for anti-Flag monoclonal antibody binding with GLP1R-Nanodisc is 3.549ng/mL. [Right] Human GLP1R-Nanodisc, Flag Tag on SDS-PAGE.参考文献：[1] Huang, KP., Acosta, A....
3.Rui Wei et al. Antagonistic Glucagon Receptor Antibody Promotes α-CellProliferation and Increases β-Cell Mass in Diabetic Mice. iScience (2019) 4.May-Yun Wang el al. Glucagon blockade restores functional β-cell massin type 1 diab...
3.Rui Wei et al. Antagonistic Glucagon Receptor Antibody Promotes α-CellProliferation and Increases β-Cell Mass in Diabetic Mice. iScience (2019) 4.May-Yun Wang el al. Glucagon blockade restores functional β-cell massin type 1 diabetic mice and enhances function of human islets. PNAS (2021...
[9]Pyke, C., Heller, R.S., Kirk, R.K., Orskov, C., Reedtz-Runge, S., Kaastrup, P., et al. 2014. GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody. Endocrinology 155:1280-1290. ...
胰高血糖素样肽-1受体(Glucagon-like peptide-1 receptor,GLP-1R),是一种膜上的G蛋白偶联受体(GPCR)。人的GLP-1受体(hGLP-1R)基因位于人染色体6p21。结构上,典型特征是具有一个相对比较大的胞外域(ECD)和有α-螺旋束构成的7次跨膜核心域(TMD)(图1)[1]。该受体的N端胞外段长116个氨基酸残基,含有3对二...
图1 胰高糖素原的组织选择性加工示意图 PCSK1:激素前转化酶1/3;PCSK2:激素前转化酶2;NTS:孤束核;GRPP:肠高血糖素相关肽;IP-1:中间肽-1;IP-2:中间肽-2;MPGF:主要胰高糖素原片段;GLP-1:胰高糖素样肽-1;GLP-2:胰高糖素样肽-2。 GCG基因在胰腺、小肠和大脑的表达均受同一个启动子的调控,从相...