Glioblastoma (GBM) develops in the complex tumor microenvironment (TME) of the brain. The GBM TME consists of extracellular matrix (ECM), interstitial fluid, and non-neoplastic cells, including both immune and non-immune cells that are compartmentalized in anatomically-distinct regions referred to as...
in in vitro experiments, to separate effects, check new scientific hypotheses, quantify the effect of each parameter or predict the outcome inwhat ifsituations. To overcome this limitation, a good possibility is combining the potential of new in vitro assays with the quantitative power and versatili...
One of the technicians gave us a sample of the plastic that was heated up so that Amber (and us) knew what to expect. And quite honestly, when she gave me my little piece, oh man was that sucker hot! I’m thinking to myself, how is Amber going to be able to lay there with ...
was dropped by drilling a small hole through the eggshell into the air sac and a 1 cm 2 window was cut in the eggshell above the cam. metformin and q54 were incubated from day 9 to 19 of embryo development, being administered every 2 days. at the end of the treatment the ...
These malignant attractors can either pre-exist hidden within the landscape or emerge due to genetic and epigenetic alterations. In both scenarios, the attractors are undesirably reached due to the inherent stochastic fluctuations of biological systems at the biomolecular scale [18]. Using the ...
Int6 Inhibition Induces Cell Cycle Arrest and G0 Transition In order to explain the decreased proliferation with Int6 inhibition, we first looked at cell cycle and cell cycle arrest in human GBM cells. Using PI/Ki67 staining and flow cytometry, we analyzed cell cycle and cells in G0. We ...