3 结果与分析 3.1 GlRTH 基因的扩增检测和测序结果 为了获得珊瑚菜 GlRTH 基因全长 ORF 序列, 通过转录组已有 GlRTH 序列设计引物,对珊瑚菜 GlRTHORF 序列进行 PCR 扩增以及克隆,测序,如图 1 电泳图 GlRTH 电泳条带位置与转录组 GlRTH A Marker 5000 bp 3000 bp 2000 bp 1500 bp 1000 bp 750 bp 500...
DNA Marker(300-5000bp) 100次 次黄嘌呤 NHS-Biotin 68-94-0 62669-70-9 Rhodamine 123 罗丹明123 谷胱甘肽-S-转移酶单克隆抗体 ARB11585 人基质裂解素(ST1)ELISA检测服务 Human stromelysin-1,st1 ELISA KIT ARB11045 人青少年2型血色素沉着症/幼年型血色病相关蛋白2(HFE2)代做ELISA实验 Human hemochromatos...
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The size of the amplification products was estimated by 100 DNA ladder or 5000 bp DNA marker (Takara, Kyoto, Japan). The gels were photographed using the gel documentation system (Bio-Rad, Hercules, CA, USA), only clear and scorable DNA bands were considered. Statistical analysis Induction...
Circular RNAs (circRNAs) are a newly discovered type of biological molecule that belongs to the noncoding RNA family. Abundant evidence has shown that circRNAs are involved in the progression of various cancers. However, the particular functions of circR
Free radicals play a major role in gliomas. By combining immuno-spin-trapping (IST) and molecular magnetic resonance imaging (mMRI), in vivo levels of free radicals were detected within mice bearing orthotopic GL261 gliomas. The nitrone spin trap DMPO (5,5-dimethyl pyrroline N-oxide) was admi...
图7是ejagl17基因过表达载体构建酶切电泳图。其中,a是pbi121载体酶切电泳图,m为dl15000dnamarker,1为双酶切后的pbi121载体;b是ejagl17过表达载体pbi121-ejagl17的双酶切验证电泳图,m为dl15000dnamarker,1为双酶切后的pbi121-ejagl17载体。 图8是6转基因前后拟南芥的开花时间照片。其中,与未转基因野生型拟...
一旦分离,可将DNA放入表达载体中,然后将所述表达载体转染入在其它情况 中不生成免疫球蛋白蛋白质的原核或真核宿主细胞,诸如大肠杆菌细胞、酵母(毕赤酵母 (Pichia)、酵母(Saccharomyces))、猿COS细胞、中国仓鼠卵巢(CHO)细胞、骨髓瘤细胞 (NS0)、昆虫或植物细胞中,以在重组宿主细胞中获得抗体或抗体所衍生的抗原结合...
Immunohistochemical analysis of O6-methylguanine-DNA methyltransferase (MGMT) protein expression as prognostic marker in glioblastoma patients treated with radiation therapy with concomitant and adjuvant Temozolomide[J] . Samar Galal Younis,Rasha Abd El-Ghany Khedr,Safinaz Hamdy El-Shorbagy.Journal of the ...
1-5:质粒p HPS9-EYFP DNAM:λDNA/HindⅢMarker 1:质粒pEYFP EcoRⅠ/BamHⅠ双酶切2:质粒pHPS9-EYFP EcoRⅠ/BamHⅠ双酶切 3 结论 利用DNA重组技术,成功地将黄绿荧光蛋白基因(EYFP)重组到大肠杆菌-枯草芽孢杆菌穿梭质粒表达载体pHPS9上,获得了含EYFP基因的大肠杆菌-枯草芽孢杆菌穿梭质粒表达载体pHPS9-EYFP,从而...