主营产品:DNA提取试剂盒,RNA提取试剂盒,蛋白提取试剂盒,PCR试剂,动物红细.. 公司地址:紫雀路33号院3号楼3516 进入商铺收藏商铺 产地:北京 品牌:百奥莱博 保质期:长期 货号:GL0792 保存条件:冷藏 GL0792型0.5%焦油紫染色液 编号:GL0792 规格:50ml
To identify wild rice genes underlying the natural variations in grain length (GL) and 1000-grain weight (TGW), we constructed a set of chromosome segment substitution lines (CSSLs) with anindicavariety ‘9311’ as the recurrent parent and theO.rufipogonas the donor parent. One major QTL for...
2b), and a near-isogenic line (NIL) of qGL12 was developed with marker-assisted selection (Fig. 1a, c). We tested the expression levels of these three genes in young panicles of the NIL and 9311, among them, expression levels of LOC_Os12g39640 and LOC_Os12g39650 were significantly ...
It is recognized that the tumor microenvironment (TME) plays a critical role in the biology of cancer. To better understand the role of immune cell components in CNS tumors, we applied a deconvolution approach to bulk DNA methylation array data in a larg
. RNA was quantified using a NanoDrop. DNase digestion was performed to prevent contamination from genomic DNA. cDNA synthesis was performed with iScript cDNA synthesis kit (BioRad). Quantitative real-time PCR (QPCR) using SsoFast EvaGreen Supermix (BioRad) was performed on a C1000 Thermal ...
In Situ Detection of Nuclear DNA Fragmentation Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) was performed on paraffin sections as described previously.41 Sections were double-labeled with antibodies to CNPase or GFAP using AEC or DAB for color development. Sections...
As for purines, the conversion steps go from IMP to ATP and dATP which, in a similar manner as pyrimidines, can serve as sub- strate for the synthesis of RNA and DNA, respectively. While PRPP is an important metabolic intermediate, in particular for the biosynthesis of nucleotides [73],...
γ-H2AX staining were used to indicate double-stranded DNA damage and apoptosis [25]. A proprietary image analysis system was used to quantify the number of positive cells over the total number of cells and the average signal emission/nucleus. Western blot...
Plagl1 and Sox9 did not induce gliogenesis in wildtype animals, but nonetheless activated expression of the Müller marker P27Kip1 in Lhx2-deficient cells. Finally, Sox2, Sox8, and Sox9 promoted amacrine cell formation in Lhx2-deficient cells, but not in wildtype retinas. These findings ...
We also analyzed the intracellular domain (ICD) ofNotch1. Following ligand binding, the Notch intracellular domain (NICD) is cleaved and transported to the nucleus, where it forms an active transcriptional regulatory complex with the DNA-binding protein RBPJ and the co-activator MAML, thereby media...