Nano-Traps consist of VHHs, also termed Nanobodies, coupled to beads. Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound Nano-Traps for IP: No heavy & light antibody chains Stringent washing conditions Low host cell protein background Very efficient...
GFP-Trap应用于拟南芥IP和ChIP的操作方法 ◀ 用GFP-trap从表达sim3-GFP的转基因拟南芥提取物中 免疫沉淀sim3-GFP。三个重复反应(1,2,3)。每个反应中取 5ul的beads进行SDS-PAGE和银染色。红色三角形表示 sim3-GFP(约94 kDa)。 Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune ...
Pre-clearing is an optional step to remove proteins or DNA which bind non-specifically to the solid-phase support. It includes the incubation of the cell extract with plain beads (e.g.binding control agarose beads) before performing the actual immunoprecipitation experiment with the GFP-Trap. Aft...
TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads. Description Immunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to ...
Nano-Trap:与agarose beads或者magnetic beads结合,在免疫沉淀或蛋白质相互作用的实验中,可高效抓取或纯化GFP或RPF融合蛋白。 (优点:三好三多) 优点一:抗体好---Nano-Traps采用的抗体是基于alpaca的单域抗体片段(VHHS);具有分子量小(15Kda)、极端稳定(70℃仍保持稳定),高亲和力(高特异性性结合GFP蛋白),无普通抗...
TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.
Magnetic beadsProtein-protein interactionProtein-protein interactions play a vital role in the cellular physiology of living organisms. Among several available approaches, co-immunoprecipitation (co-IP) has emerged as a reliable method to investigate such interactions. The underlying principle is to ...
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. The ChromoTek GFP-Trap® Agarose is a ready-to-use reagent. Specificity GFP and GFP derivates Applications IP, CoIP, ChIP, RIP Conjugate Agarose Type...
The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads. DescriptionImmunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to magnetic agarose ...
产品描述: 与agarose beads结合particle size ~ 80 µm 存储缓冲液:20% EtOH 储存方法:室温运输,收到后4℃储存一年。请勿冰冻。 ChromoTek 代理|GFP-Trap®_A 20 rxns (500µl)大量供应"