Nano-Trap:与agarose beads或者magnetic beads结合,在免疫沉淀或蛋白质相互作用的实验中,可高效抓取或纯化GFP或RPF融合蛋白。 (优点:三好三多) 优点一:抗体好---Nano-Traps采用的抗体是基于alpaca的单域抗体片段(VHHS);具有分子量小(15Kda)、极端稳定(70℃仍保持稳定),高亲和力(高特异性性结合GFP蛋白),无普通抗...
Nano-Traps consist of VHHs, also termed Nanobodies, coupled to beads. Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound Nano-Traps for IP: No heavy & light antibody chains Stringent washing conditions Low host cell protein background Very efficient...
Pre-clearing is an optional step to remove proteins or DNA which bind non-specifically to the solid-phase support. It includes the incubation of the cell extract with plain beads (e.g.binding control agarose beads) before performing the actual immunoprecipitation experiment with the GFP-Trap. Aft...
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. The ChromoTek GFP-Trap® Agarose is a ready-to-use reagent.
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. The ChromoTek GFP-Trap® Agarose is a ready-to-use reagent. Description The GFP-Trap Agarose Kit contains GFP-Trap Agarose, lysis, wash, and elution ...
The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads. DescriptionThe GFP-Trap Magnetic Agarose Kit contains GFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunop...
GFP-trapMagnetic beadsProtein-protein interactionProtein-protein interactions play a vital role in the cellular physiology of living organisms. Among several available approaches, co-immunoprecipitation (co-IP) has emerged as a reliable method to investigate such interactions. The underlying principle is ...
拟南芥的IP实验Protocol拟南芥的ChIP实验ProtocolProteintech旗下品牌GFP-Trap应用于拟南芥IP和ChIP的操作方法◀ 用GFP-trap从表达sim3-GFP的转基因拟南芥提取物中免疫沉淀sim3-GFP。三个重复反应(1,2,3)。每个反应中取5ul的beads进行SDS-PAGE和银染色。红色三角形表示sim3-GFP(约94 kDa)。Formation of NPR1 Conde...
产品描述:与agarose beads或者magnetic beads结合,在免疫沉淀或蛋白质相互作用的实验中,可高效抓取或纯化GFP或RPF融合蛋白。 Chromotek的Nano-Trap®基于alpaca的单域抗体片段(VHHS) 小型,分子量在15Kda 极端稳定,70℃仍保持稳定 高亲和力,高特异性性结合GFP蛋白GFP-Trap® 产品特点(高稳定性、高亲和力) 特异性...
ChromoTek公司GFP-Trap与RFP-Trap产品说明 德国ChromoTek公司成立于2008年,致力于细胞生物学和蛋白质组学的研发与研究,公司拥有强大的研发团队,有先进的生物制剂,主要主要使命是花费较少的时间和成本,使客户获得得高质量的实验数据。 GFP-Trap® —— 值得信赖的科研工具: 产品描述: 与agarose beads或者magnetic ...