Tagged proteins can be used for purposes such as immunoprecipitation, microscopy, protein purification, Western blotting, protein arrays, etc. Proteins fused to GFP are called GFP-tagged proteins or GFP-fusion proteins. GFP-tagged proteins are often used for fluorescence microscopy, immunoprecipitation,...
It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.注意事项:1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体...
As nanobodies are powerful tools for the purification of GFP-tagged proteins and there are many commercialized nanobody resin products used for purification, we attempted to produce a fusion nanobody with heightened affinity to GFPuv for use in purifying protein with improved yield. Repeated (GGGGS...
SDS, e.g. SDS sample buffer, is a very effective way to elute thebound GFP-taggedprotein. The elution results in denatured GFP-fusions. 0.2 M glycine pH 2.5Alternatively you may elute with glycine at pH 2.5. It isrecommended to repeat thiselution step as the pH shift elution works incomp...
(excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without ...
enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence ener...
Description 10mg/ml magnetic beads in specific protective buffer ~200nm Superparamagnetic Anti-GFP mouse monoclonal antibody IgG1 Approximately 150kDa ≥0.6mg GFP antibody per ml beads ≥ 0.6mg GFP‐tagged fusion protein per ml beads GFP-Protein, Protein-GFP SDS‐PAGE loading buffer, or acid ...
(Novagen). pET28a appends an N-terminal 6XHis-tag for protein purification. All constructs were verified by automated DNA sequencing. N-terminal 6XHis-tagged Drp1-wt, mEGFP, mEGFP-Drp1, and Drp1-mEGFP were expressed recombinantly inE. coliand purified to apparent homogeneity as previously ...
Binding capacity ≥ 0.6mg GFP‐tagged fusion protein per ml beads Specificity GFP-Protein, Protein-GFP Elution method SDS‐PAGE loading buffer, or acid elution.Note: If elute with SDS-PAGE loading buffer, the light (~25kDa) and heavy (~50kDa) chain of GFP antibody will be denatured and ...
标签抗体 •GFP (Green Fluorescent Protein)•Renilla GFP •RFP (Red Fluorescent Protein)•DDDDK-tag •HA-tag •Myc-tag •His-tag GFP (Green Fluorescent Protein)•GFP多克隆抗体使用文献多达500篇以上!可以适用于各种实验应用: WB, IP, IC, FCM •GFP单克隆抗体(克隆: 1E4)可以适用于...