a bio-sensor (pH, calcium) GFP derivatives Many fluorescent proteins are based on the GFP sequence. These fluorescent proteins are genetically engineered and have different properties such as different excitation/emission spectra, photo or pH-stability, folding properties, half-time etc. Here is a ...
This final round of screening lead to the identification of a highly pH-stable protein (sfGFP-G4R, N149Y, I167T, I188V, Q204H). To validate that our screen lead to a pH stability improvement, we tested the change in excitation ratio between the protonated (405 nm) and ...
- Fast maturation at a wide range of temperatures - High pH-stability and photostability - Proven suitability to generate stably transfected cell lines - Destabilized variant is available - Recommended for gene expression analysis and cell and organelle labeling ...
Stability:Samples are stable for up to twelve months from date of receipt at -70 ℃ Predicted N terminal: Met Molecular Mass:The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7 kDa. It migrates as an 35 kDa band in SDS-PAGE under...
Thermal stability of recombinant green fluorescent protein (GFPuv) at various pH values The thermal stability of the recombinant green fluorescent protein (GFPuv) expressed by Escherichia coli cells and isolated by three-phase partitioning ext... TCV Penna,M Ishii,JO Cholewa - 《Applied Biochemistry ...
. The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation, high stability at a wide range of pH (pH 4-12), and does not require any additional cofactors or substrates. The copGFP protein has very bright fluorescence that exceeds at least 1.3 times the ...
Unique Stability of the ChromoTek GFP-Trap:GFP Complex GFP-Trap as affinity tag How to plan an Immunoprecipitation using the GFP-Trap Efficient purification of GFP-tagged membrane proteins Split Fluorescent Protein Technology (PDF) Trouble shooting ...
Unique Stability of the ChromoTek GFP-Trap:GFP Complex Application notes GFP immunoprecipitation of Arabidopsis thaliana plant samples Elution of GFP-fusion protein from the GFP-Trap On-bead digest protocol for mass spectrometry Ubiquitination of GFP-tagged proteins ...
Marina Ishii,Juliana Sayuri Kunimura,Hélio Tallon Jeng,Thereza Christina Vessoni Penna,Olivia Cholewa.Evaluation of the pH- and thermal stability of the recombinant green fluorescent protein (GFP) in the presence of sodium chloride[J]. Applied Biochemistry and Biotechnology .2007(1-12)...
Unique Stability of the ChromoTek GFP-Trap:GFP Complex Efficient purification of GFP-tagged membrane proteins GFP-Trap Specificity Fluorescent protein specificity table Application notes On-bead digest protocol for mass spectrometry Capture Surface for Biacore assays ...