Monocle2将在内部执行所有必需的标准化步骤,自己提前进行归一化处理可能会破坏Monocle的一些关键步骤。 Monocle2也可以通过importCDS和exportCDS函数将其他格式的数据导入为CellDataSet对象,或将CellDataSet对象导出为其他格式。 # Where 'data_to_be_imported' can either be a Seurat object or an SCESet.# 将其他格...
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PS- If you have single-cell RNASeq data, I would recommend using some single-cell specific packages such as seurat in R or ScanPy in python to do your analysis. They include everything from data-processing, clustering and generating plots such as these within them. It will make your life...