将integrated作为默认assay进行PCAUMAP等分析 6 Change default assay to "RNA"; normalize then generate FeaturePlots and perform differential expression analysis 将默认assay设置为RNA或者SCT进行鉴定marker及差异基因等分析,完成后续处理 参考材料: https://github.com/satijalab/seurat/issues/1836 欢迎关注微信公众...
SEURAT: Visual analytics for the integrated analysis of microarray data 来自 Semantic Scholar 喜欢 0 阅读量: 63 作者:A Gribov,M Sill,S Lück,F Rücker,Antony R. Unwin 摘要: Background In translational cancer research, gene expression data is collected together with clinical data and genomic ...
@zihengxuwuHello,your script imported the RNA counts not the integrated data.But I have to use the batch integrated data of Seurat.In addition, monocle3 is not the final edition,and its result trajectory is really not available for me.So I still use monocle 2 to do it. ...
(http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.SoftwareSEURAT: Visual analytics for the integrated analysis of microarray dataAlexander Gribov†1, Martin Sill†2, Sonja Lück3...
Hello. Is it possible to add new metadata to categorize a group of samples? I usually use AddMetaData() to add sample identities or groupings to each of the samples/seurat objects, but I don't know how to do it in an integrated object. I...
By default,DotPlotuse the data stored indataslot of current active assay. I found your subset object has already with default assay set toRNA: RLU.combined.small<-readRDS("RLU.combined.small.Rds")#> dim(RLU.combined.small)#Loading required package: Seurat#[1] 25843 20#> DefaultAssay(RLU....
Hello! I am wanting to run AddModuleScore() to an integrated dataset (I used the non-SCTransform pipeline), but it returns the following error: Error in sample.int(length(x), size, replace, prob) : cannot take a sample larger than the po...
Error in scaling with cell cycle genes regression an integrated object#4552 MartaBenegasopened this issueMay 31, 2021· 3 comments Assignees Comments Copy link MartaBenegascommentedMay 31, 2021 Hi Seurat Team! I've tried to perform an integration workflow with cell cycle genes regression as speci...