将integrated作为默认assay进行PCAUMAP等分析 6 Change default assay to "RNA"; normalize then generate FeaturePlots and perform differential expression analysis 将默认assay设置为RNA或者SCT进行鉴定marker及差异基因等分析,完成后续处理 参考材料: https://github.com/satijalab/seurat/issues/1836 欢迎关注微信公众...
Error: package or namespace load failed for ‘Seurat’ in loadNamespace#3532 Closed shwetajoharimentioned this issueOct 6, 2022 Integrated Seurat Object isnt converting to Monocle 3 object.#6500 Closed Sign up for freeto join this conversation on GitHub. Already have an account?Sign in to comm...
Hello. Is it possible to add new metadata to categorize a group of samples? I usually use AddMetaData() to add sample identities or groupings to each of the samples/seurat objects, but I don't know how to do it in an integrated object. I previously integrated samples 1-10 and already...
(http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.SoftwareSEURAT: Visual analytics for the integrated analysis of microarray dataAlexander Gribov†1, Martin Sill†2, Sonja Lück3...
By default,DotPlotuse the data stored indataslot of current active assay. I found your subset object has already with default assay set toRNA: RLU.combined.small<-readRDS("RLU.combined.small.Rds")#> dim(RLU.combined.small)#Loading required package: Seurat#[1] 25843 20#> DefaultAssay(RLU....
Hi Seurat Team! I've tried to perform an integration workflow with cell cycle genes regression as specified in #3665 but it raises an error that I don't know how to solve. Here's my code: suppressPackageStartupMessages(suppressWarnings(l...