This unit describes the use of the 5-nuclease allelic discrimination assay, or TaqMan, for genotyping with single nucleotide polymorphisms (SNPs). In this method, the region flanking the polymorphism, typically 100 to 150 bp, is amplified in the presence of two probes, each specific for one ...
With TaqMan®technology allele-specific probes are utilized for quick and reliable genotyping of known polymorphic sites. TaqMan assays are robust in genotyping multiple variant types, including single nucleotide polymorphisms, insertions/deletions, and presence/absence variants. To query a single bi-alle...
includes two differentially labeled, allele-specific TaqMan MGB probes and a PCR primer pair that uniquely amplify and provide unmatched specificity for the allele of interest. Choose an assay from one of more than 17 million predesigned assays, or customize your own using our Cust...
‹Drug Metabolism Genotyping Analysis Using TaqMan Assays Single Tube Drug Metabolism Genotyping TaqMan® Assays TaqMan® Drug Metabolism Assay Sets Life Technologies offers a collection of TaqMan™ Drug Metabolism Genotyping Assays for pharmacogenomics applications. Th...
(agaedigk@cmh. edu) SNP genotyping using TaqManH technology: the CYP2D6*17 assay conundrum Andrea Gaedigk1,2, Natalie Freeman3, Toinette Hartshorne4, Amanda K. Riffel1, David Irwin3, Jeffrey R. Bishop5, Mark A. Stein6, Jeffrey H. Newcorn7, Lazara Karelia Montane´ Jaime8, Mariana ...
SNPs are a simple form of genetic variation that can influence an individual’s susceptibility to disease or response to drugs, or trace the inheritance of specific genes and traits of interest. TaqMan SNP Genotyping assays combine gold standard ...
Taq DNA polymerase’s 5’-nuclease activity is used in the Taqman assay for SNP genotyping. The Taqman assay is performed concurrently with a PCR reaction and the results can be read in real-time as the PCR reaction proceeds (McGuigan & Ralston 2002). The assay requires forward and reverse...
The specificity of the RT-qPCR assays was assessed by testing HAV RNA of each subtype at a concentration of 5 × 104 genome copies/assay with all the subtype-specific RT-qPCR assays. As shown on Table 4, detection of the specific target was observed for all assays with Ct values comprised...
(agaedigk@cmh. edu) SNP genotyping using TaqManH technology: the CYP2D6*17 assay conundrum Andrea Gaedigk1,2, Natalie Freeman3, Toinette Hartshorne4, Amanda K. Riffel1, David Irwin3, Jeffrey R. Bishop5, Mark A. Stein6, Jeffrey H. Newcorn7, Lazara Karelia Montane´ Jaime8, Mariana ...
TaqMan® Drug Metabolism Genotyping Assays offer the broadest menu of drug metabolism enzyme (DME) targets on the market, offering 2,700 unique assays that detect polymorphisms in 221 genes for high-value DMEs and a...