1 These entered the genome via germ cells as transposable element DNA, first described in maize by Barbara McClintock.13 Interestingly, we have learned that approximately 8% of the intronic base sequences are the products of human endogenous retroviruses (HERVs). The segments are DNA-based ...
These two lines which had chromosome pairs 17 (CS-B17) and 25 (CS-B25) replaced by the respective pair from doubled-haploid line 3-79 were used for pollen DNA analyses. The primer extension pre-amplification (PEP) protocol was also successful on the pollen grains. The use of pollen is ...
Genomic DNA exhibits high heterogeneity in terms of its dynamic within the nucleus, its structure and functional roles. CRISPR-based imaging approaches can image genomic loci in living cells. However, conventional CRISPR-based tools involve expressing constitutively fluorescent proteins, resulting in high...
Using matched whole-genome sequencing data, we associated several categories of RNA alterations with germline and somatic DNA alterations, and identified probable genetic mechanisms. Somatic copy-number alterations were the major drivers of variations in total gene and allele-specific expression. We ...
DNA double-strand breaks can lead to chromosomal rearrangements at the first mitosis after exposure to the DNA strand-breaking agent. The evidence suggests a number of different pathways for DNA double-strand break rejoining in mammalian cells, but it is unclear what factors determine the fate of...
(especiallyP. orbignyanus) presented a very low frequency of REs in general. Among the Pleuronectiformes, the genome ofP. orbignyanushas a lower proportion of DNA transposons and a higher proportion of long (LINEs) and short (SINEs) interspersed nuclear elements. We also studied the ...
Cervical cancer (CC) causes more than 311,000 deaths annually worldwide. The integration of human papillomavirus (HPV) is a crucial genetic event that contributes to cervical carcinogenesis. Despite HPV DNA integration is known to disrupt the genomic arc
DNA double-strand breaks can lead to chromosomal rearrangements at the first mitosis after exposure to the DNA strand-breaking agent. The evidence suggests a number of different pathways for DNA double-strand break rejoining in mammalian cells, but it is unclear what factors determine the fate of...
DNaseI digest, and combined analyses of epigenetic variation, chromatin immunoprecipitation, and DNase-seq data27. The greatest levels ofcis- andtrans-eQTL enrichment relative to all variants in the genome were found for putatively deleterious variants, followed by missense and synonymous variants (Fig...
(45.0%). DNA Polymerase Epsilon (POLE) at 12q24.33, where was also considered lost in HCC, lost one copy in 40 patients (23.7%). In addition, important DNA methylation factor DNMT3A (2p24.1) and chromatin methylation factor EZH2 (7q35) also show loss in 20 (11.8%) and 19 (11.24%)...