We outline in this short monograph the steps needed to implement a methodology that allows for genome-wide genetic screening in the central nervous system of mice to study both normal and degenerative disease gene function.doi:10.1007/978-3-319-60192-2_3Mary H. Wertz...
Here we conducted a genome-wide CRISPR screening combined with an optimized ATAC-see protocol to identify genes that modulate global chromatin accessibility. In addition to known chromatin regulators like CREBBP and EP400, we discovered a number of previously unrecognized proteins that modulate chromatin...
Fig. 2: Host dependency factors and pathways of SARS-CoV-2 in lung cells revealed by genome-wide LOF screening. a, Protein–protein interaction network for top 100 enriched hits identified in the CRISPR LOF screen based on STRING analysis. Solid lines between genes indicate direct interaction, ...
Design target assays for population health screening Run high-powered genome-wide association studies Predict genetic risk of disease See how> Plant and animal genomics The Gencove platform unifies high- and medium-density genotyping into a single scalable, flexible, and low-cost assay. ...
迄今为止,全基因组关联研究(以下简称GWAS)发展已有二十多年了。这二十多年间,随着样本数的越来越大以及基因芯片的物美价廉,GWAS也得到了更多的发展,科学家们发现了大量和人类疾病以及其它表型相关联的基因,在此基础上,GWAS还推动了孟德尔随机化和多基因风险评分的发展与应用。可以说,GWAS是现代遗传学的重要组成部分,...
Thus, our unbiased genome-wide screening approach uncovered regulation of DNA replication as a major genetic signature upon RSV treatment across different cell lines and different sgRNA libraries. Network Analysis of Chemical-Genetic Interactions with RSV, PTS, and HU Reveals Critical Modules Implicated ...
A genome-wide RNAi screen was carried out using dsRNAs fromDrosophilaHeidelberg 2 (HD2) library, covering about 98% of the annotatedDrosophilagenome [27,28]. Importantly, dsRNAs against known trxG members (trx, ash1) and specific reporter gene (F.Luc) were used as positive controls, whereas...
Several of these regions have been observed in other genomewide screens and linkage or association studies, for asthma and related phenotypes. These results were used to develop a conceptual model to delineate asthma-susceptibility loci and their genetic interactions, which provides a promising basis ...
Functional genomic screening using the CRISPR-Cas9 system has emerged as a powerful approach to associate genetic perturbations with a distinct phenotype in cancer cells [57]. In particular, genome-wide loss-of-function screens in established cancer cell models allow for the identification of genetic...
To identify host factors essential for coronavirus infection, we performed genome-wide functional genetic screens with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human coronavirus 229E. These screens uncovered virus-specific as well as shared host factors, including TMEM41B and ...