We outline in this short monograph the steps needed to implement a methodology that allows for genome-wide genetic screening in the central nervous system of mice to study both normal and degenerative disease gene function.doi:10.1007/978-3-319-60192-2_3Mary H. Wertz...
4), enabling near genome-wide representation required for comprehensive forward genetic screening. Genome-wide CRISPR/Cas9 screening for modulators of cardiotoxicity Cardiotoxicity remains among the most significant impediments for new drug approval, particularly among many anti-cancer agents37. While a ...
Genome-wide analysis of gene expression is the study of transcription at a genomic scale, also known as transcriptomics. Typically relying on data from microarrays or high throughput sequencing, it can determine what genes or transcript isoforms are enriched in a particular cell or tissue type, con...
A genome-wide RNAi screen was carried out using dsRNAs fromDrosophilaHeidelberg 2 (HD2) library, covering about 98% of the annotatedDrosophilagenome [27,28]. Importantly, dsRNAs against known trxG members (trx, ash1) and specific reporter gene (F.Luc) were used as positive controls, whereas ...
A Genome-wide Framework for Mapping Gene Regulation via Cellular Genetic Screens Cell, Volume 176, Issue 6, 7 March 2019, Pages 1516 Molly Gasperini, Andrew J. Hill, José L. McFaline-Figueroa, Beth Martin, Seungsoo Kim, Melissa D. Zhang, Dana Jackson, Anh Leith, Jacob Schreiber, William...
Genome-wide screening for MHC-I Two replicates of each doxycycline-inducible Cas9 cell line were independently transduced with Brunello library lentivirus such that transduction efficiency was between 15%–25%. sgRNA coverage was maintained throughout the experiments at > 500 copies of each sgRNA (...
In addition, varied fertility of F1s (from less than 4.7% to greater than 95%) and the diverse morphological traits also indicated wide genetic diversity in A. duranensis[12, 69]. Univalents, laggards, and multivalents can all be detected in intraspecific A-genome and interspecific A- by...
the two target sites were incorporated into PCR forward and reverse primers, respectively. The PCR fragment was amplified from pCBC-DT1T2 for dicot targets or pCBC-MT1T2 for monocot targets with two long primers or four shorter primers, among which two forward or two reverse primers were...
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In addition, mutual introgression within the Guizhou chicken breeds was also suggested by the results obtained from the ADMIXTURE analysis. Furthermore, the genome wide genetic differentiation analysis was carried out to detect the admixture or migrant events happening between the breeds. Consistently, ...