Investigations of such cell-to-cell l-lactate shuttles would be facilitated by a genetically encoded fluorescent biosensor that would enable high resolution spatially and temporally resolved imaging of the extracellular l-lactate concentration. Genetically encoded fluorescent biosensors are powerful tools for...
Genetically encoded biosensors based on fluorescent proteins (FPs) are powerful tools for tracking analytes and cellular events with high spatial and temporal resolution in living cells and organisms. Compared with intensiometric readout and ratiometric readout, fluorescence lifetime readout provides ...
A protein-based genetically encoded fluorescent sensor usually consists of a fluorescent protein and a sensing domain21. When a target molecule binds to the sensing domain, it changes the conformation of the sensor, resulting in the change of the sensor’s fluorescent properties, for example brightn...
Genetically encoded fluorescent biosensors can capture anions in action across time and space dimensions with microscopy. The firsts of such technologies were reported more than 20 years for monoatomic chloride and polyatomic cAMP anions. However, the recent boom of anion biosensors illuminates the ...
E. (2009). Genetically encoded biosensors based on engineered fluorescent proteins. Chem. Soc. Rev. 38 2833–2841 10.1039/b907749a [ Cross Ref ]Frommer WB, Davidson MW, Campbell RE (2009) Genetically encoded biosensors based on engineered fluorescent proteins. Chem Soc Rev 38:2833–2841 ...
The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this obligate intracellular protozoon. FPs like the green fluorescent protein (GFP) enabled to explore protein localization, promoter activity as well
The genetically encoded fluorescent biosensors described above have several applications but the affinity of the sensor and how it reacts to pH fluctuations should be taken into consideration before use. The sensors that have an affinity for NADH were SoNar, Peredox and RexYFP. Peredox has the ...
For over a decade, several attempts have been made to create genetically encoded, fluorescent biosensors that can detect changes in cAMP and report these changes through alterations in fluorescence. Despite strenuous efforts, involving many different design strategies, over the course of more than ...
Genetically encoded fluorescent biosensors represent a new tool for investigation of biological processes including redox ones. Biosensors allow real-time monitoring of messengers, metabolites, and enzyme activities in live systems of different levels of complexity from cultivated cells to transgenic animals....
In this chapter we describe a method to monitor phosphoinositides dynamically by transfection of fluorescently tagged probes (biosensors) into cultured macrophages. These biosensors are based on the fusion of phosphoinositide-binding protein domains with fluorescent proteins. Some specifications for live cell...