et al. Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2. Sci. Rep. 7, 11999 (2017). Article PubMed PubMed Central ADS Google Scholar Nielsen, S. S. et al. BioXTAS RAW, a software program for high-throughput automated small-angle X-...
Ensemble FRET experiments provide an apparent FRET efficiency (\({E}_\mathrm{f}^{\mathrm{app}}\)), which is inversely proportional to the distance between the two fluorescent proteins (FPs) in our FRET construct. When the FPs are close together,\({E}_\mathrm{f}^{\mathrm{app}}\)is ...
The review focuses mainly on the lux- and gfp-inserted whole-cell biosensors producing bioluminescence and multicoloured fluorescent proteins, which allow an easy and reproducible detection of the signals from a large number of prokaryotic and eukaryotic soil-borne microorganisms. This review also ...
The genetic marker, a green fluorescent protein gene, was used to identify the endophytic PAE-degrader Bacillus subtilis N-1. Soil and rice plants exposed to di-n-butyl phthalate (DBP) supported the colonization of the inoculated N-1-gfp strain, a finding corroborated by confocal laser ...
require activation and assembly. However, there are currently no tools to study the dynamics of large oligomeric pores spanning their activation, formation, and function in live cells. In this work, through a combination of electrophysiology, optogenetic design, live-cell fluorescent biosensing, and ...
This method specifically targets the construction of translational fusions to yield hybrid proteins, incorporating a fluorescent carboxyl-terminal domain. The fluorescent protein-encoding sequence can be strategically placed at any codon site of the target gene's mRNA for reliable reporting on gene ...
Although biosensors are a novel achievement of bioanalytical chemistry, they are not only a subject of intensive research, but also a real commercial product (Kissinger, 2005). The estimated world analytical market is about $20 billion per year of which 30 % is in the healthcare field. The ...
Using a CRISPR loss-of-function screening approach, we ascertain that the production of a flavivirus-encoded fluorescent reporter protein necessitates the involvement of diverse host factors, notably several proteins governing 60S ribosome biogenesis. Viral phenotyping indicated that two of these factors,...
(B). All samples were co-stained with the fluorescent dye BODIPY™ 493/503 and counterstained with DAPI to highlight lipid droplets and nuclei, respectively. Composite images of all x–y optical sections are shown. Cell extremities and extracellular lipidosomes are displayed in the left and ...
The images were obtained after compound treatment for 1 hr, permeabilized, stained with anti-GPR35, followed by fluorescent secondary antibody. Red: GPR35 stains; Blue: nuclei stains with DAPI. Representative images obtained from 2 independent measurements were used. treat congestive heart failure ...