Then, only in the human lineage, the ancestral PDE4DIP-NOTCH2NL fusion gene was “revived” by NOTCH2 through ectopic gene conversion. With the acquisition of exon 1 and the upstream promoter, a viable NOTCH2NL gene encoding a stable NOTCH2-related protein was created. Because no remnants ...
Chimeric antigen receptor (CAR) T cells have induced spectacular remissions and are now approved for use in patients with relapsed/refractory hematological malignancies. This has revived the interest in using CAR T cells and natural killer (NK) cells in solid tumors such as CRC, especially in the...
In (C) and (D), populations from each passage were revived from glycerol stocks in the same media condition that they were initially grown in. Mutation frequencies were calculated from Sanger sequencing of revived populations, as in (B). See also Figures S4 and S5 and Tables S6 and S9. ...
Human primary pulmonary alveolar epithelial cells (HPAEpiC) (Science Cell Research laboratories, Carlsbad, CA, USA) were revived according to the manufacturer’s instructions. A T-25 flask was coated with Poly-L-lysine (Sigma Aldrich) at a concentration of 2 µg/cm3 kept at 37 °C for 24...
(YCH) and Centre Pasteur du Cameroun (CPC) between January 2015 to March 2016. Presumptive identification of the isolates was performed in each collection site using either API 20 NE or VITEK 2. Bacteria isolates were revived on Tryptic Soy Agar (TSA) and transported to the Food and Drug ...
(YCH) and Centre Pasteur du Cameroun (CPC) between January 2015 to March 2016. Presumptive identification of the isolates was performed in each collection site using either API 20 NE or VITEK 2. Bacteria isolates were revived on Tryptic Soy Agar (TSA) and transported to the Food and Drug ...