Virulence assays in vitro (with macrophages) and in vivo (using a mouse model) similarly implicated RecA in the pathogenicity of A. baumannii . Thus, the data strongly suggest a protective role for RecA in the bacterium and indicate that inactivation of the protein can contribute to a ...
30. However, monitoring changes in intracellular NAD+at multiple subcellular locations is limited by the spectral incompatibility and selectivity of current biosensors31,32,33. Inspired by the recently developed NAD+sensor based on DNA ligase
Electrophysiological experiments and radioligand-binding assays as well as molecular modeling studies with wild-type and mutated Na+ channel proteins have provided evidence for a specific pyrethroid binding site, but the isolation of the membrane-embedded receptor remains elusive. Two types of ...
DGGE of PCR-DNA appears to be effective for this purpose~ Now we are accumulating data from DGGE of PCR-DNA using the human factor IX gene (FIX). Variations observed in FIX of 124 Japanese were presented. In the first experiment, a region between positions -1218 and -236 from the ...
In both of them we designed the DNA-binding domain to include the mutant G at position C3 of the antisense strand (Figure 1 and Supplementary Figure S1). These TALENs were initially tested in yeast Single-Strand Annealing assays. Because one of the pairs (version A) showed similar efficiency...
happens before menstruation and is believed to occur due to hypersensitivity to progesterone and to the vasoactive component in menstrual fluid such as prostaglandins. Most tests of anaphylaxis such as complement assays, serum tryptase and tests for carcinoid and phaeochromocytoma usually return negative...
Here, we identified General Control Non-Repressible 20 (GCN20), a member of subfamily F, as new factor for DNA damage repair in root growth. While gcn20–1 mutant had a short primary root with reduced meristem size and cell number, similar primary root lengths were assayed in both wild-...
C: Results from three independent MTT assays to calculate IC50 and AUC. D–G: Cell viability in UM-UC-3, J82, RT112, and 5637 cells, respectively, treated with increasing concentrations of ADI-PEG 20 for 48 hours. ∗P < 0.05 compared with untreated control, t-test. ADI-PEG 20, ...
Hypogonadism and hypopituitarism were suspected from hormon assays of urine and serum. Late replicating X chromosome was identified to be morphologically normal X chromosome, not translocated one, by both autoradiography and BUDR-acridine orange methods. Several cases with active X-autosomal translocation ...
As explained above, the immunometric assays for antigen require that the particular binding molecule be labeled with a "reporter molecule." These reporter molecules or labels, as identified above, are conventional and well-known to the art. In the practice of the present invention, enzyme labels...