The well-known assays, such as tetrazolium-based assays used for mitochondrial function monitoring, LDH for membrane permeability determination and neutral red uptake (NRU) describing lysosome function, need to be optimised due to specific properties of wide range of nanomaterials. Physicochemical ...
the labelled exosomes were resuspended in medium and incubated with CEP cells for internalization assays. The nuclei of CEP cells were stained with 4’,6-diamidino-2-phenylindole (DAPI; C1006, Beyotime, China). The uptake of labelled exosomes by CEP cells was evaluated by fluorescence microscopy...
Human fatality due to thallium poisoning: Autopsy, microscopy, and mass spectrometry assays. J. Forensic Sci. 2015, 60, 247–251. [Google Scholar] [CrossRef] Repetto, G.; Sanz, P.; Repetto, M. Comparative in vitro effects of sodium arsenite and sodium arsenate on neuroblastoma cells. ...
Usedin vitrofor detection of specific forms of DNA damage or for repair assays. Flow Cytometry Supplemental Reagents Antibody isotype controls and supplemental staining reagents for flow cytometry. Immunohistochemistry Supplemental Reagents Antigen retrieval reagents, cell and tissue staining kits, staining re...
However, similar trends can also be observed by ChIP-seq analysis for other enhancer marks (H3K4me1) and mediator proteins or by other methods, including DNase I hypersensitivity assays and Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) analysis [20,39]. Although the super...
Electrophysiological experiments and radioligand-binding assays as well as molecular modeling studies with wild-type and mutated Na+ channel proteins have provided evidence for a specific pyrethroid binding site, but the isolation of the membrane-embedded receptor remains elusive. Two types of ...
It is common to be asked to write an IELTS apology letter for General Training writing task 1. You might be given a formal or informal apology letter. You must use the correct style and tone to get a high band score. Always decide what type of letter you must write before you start ...
Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother. 2000;44(2):231–238. Article PubMed CAS Google Scholar ...
C: Results from three independent MTT assays to calculate IC50 and AUC. D–G: Cell viability in UM-UC-3, J82, RT112, and 5637 cells, respectively, treated with increasing concentrations of ADI-PEG 20 for 48 hours. ∗P < 0.05 compared with untreated control, t-test. ADI-PEG 20, ...
A-B, Target DNA binding assay A, resolved by native polyacrylamide gel electrophoresis (PAGE) mobility shift assays and B, quantification with WT-normalized dissociation constants. C-D, Quantified DNA cleavage rates (detection limit for kcleave set at 10 min′) displayed as a C, heatmap and ...