1)离体基因治疗(Ex vivo)是直接取自异体正常细胞或患者自身的病变细胞,体外通过基因导入的方式修饰细胞,将修饰后的细胞体外扩增后回输到病人体内,从而达到治疗疾病的目的。 2)在体基因治疗(In vivo)则是利用非病毒或病毒载体直接将治疗基因递送到患者体内的方式来治疗疾病。 临床试验针对个体差异和疾病类型的不同而...
Significant progress in preclinical and clinical studies has demonstrated the potential of in vivo and ex vivo gene therapy to treat various NDDs, offering a versatile and precise approach in comparison to conventional treatments. The current review describes various gene therapy approaches employed in ...
The ex vivo approach is mainly used to edit blood cells, improve cancer therapy, and treat infectious diseases. To edit cells in organs researches choose in vivo approach. For each approach, there is a fairly large set of methods, but, unfortunately, these methods are not universal in their...
gene electrotransfer; ophthalmological disorders; in vivo; ex-vivo1. Introduction The idea of gene therapy dates back more than half a century, when it was understood that nucleic acids carry genetic information [1]. Cell electroporation or electropermeabilization generally occurs when cells either ...
Recent clinical and preclinical studies have described exciting results using recombinant adeno-associated virus (AAV) vectors for therapeutic gene transfer in genetic disease. This Review explores the potential for using this form of therapy in the cont
Ex vivo and in vivo adenovirus-mediated gene therapy strategies induce a systemic anti-tumor immune defence in the B16 melanoma model. 喜欢 0 阅读量: 28 作者:B Bonnekoh,DA Greenhalgh,SH Chen,A Block,DR Roop 摘要: The Journal of Investigative Dermatology publishes basic and clinical research ...
Prieto J, Herraiz M, Sangro B, Qian C, Mazzolini G, Melero I, et al: The promise of gene therapy in gastrointestinal and liver diseases. Gut. 2003, 52 (Suppl 2): ii49-ii54. CAS PubMed PubMed Central Google Scholar Raper SE, Wilson JM: Making space for intestinal gene therapy. ...
Engineered Cells and Ex Vivo Gene Transfer Ever since the development of recombinant DNA technology, the promise of these methods for dramatically improving medicinal therapy has been vigorously championed. The possibility to interfere with gene expression in dysfunctioning cell... C Lundberg - Humana...
Genetherapy falls into two main categories. Ex vivo gene therapy removes cells fromthe patient, introduces new genetic material, packaged in a delivery vehiclecalled a vector, then returns the cells to the patient. Boston Children’s isusing this method for such disorders as sickle cell disease,...
(1999). In vivo and ex vivo gene therapy strategies to treat tumors using adenovirus gene transfer vectors. Cancer Chemother. Pharmacol. 43(Suppl.), S90-S99.Crystal RG.In vivo and ex vivo gene therapy strategies to treat tumors using adenovirus gene transfer vectors.Cancer Chemother. 1999...