we describe how CRISPR-Cas9 gene editing can be exploited to rapidly engineer one of the most complex megasynthase assembly lines in nature, the 2.0 MDa NRPS enzymes that deliver the lipopeptide antibiotic enduracidin. Gene editing was used to exchange subdomains within the NRPS, altering substrate...
Sign in to download hi-res image Figure 2.Gene editing. Gene editing may be initiated using a site-specific endonuclease, such as the CRISPR/Cas9 system, to produce a DNA double-strand break (DSB) at a target site in the genome. The DSB may be repaired by different DNA repair pathways...
Both CRISPR-based gene editing and transcriptional regulation benefit from multiplexing (Fig.1). By producing multiple gRNAs and a Cas protein in vivo, researchers can build layered genetic circuits that control cellular behavior or modulate metabolic pathways with the simultaneous editing, activation, a...
base editing has enabled the introduction of specific transition mutations using a Cas9 nickase (Cas9n) fused with adenine or cytosine deaminase. In the case of cytosine base editing enzymes (BEs), they use a uracil glycosylase inhibitor (UGI) to prevent base excision repair and promote C >...
CRISPR, which stands for clustered regularly interspaced short palindromic repeats, holds promise for correcting mutations in the human genome to prevent genetic disease. The gene-editing technique, done in concert with in vitro fertilization (IVF), could provide a new avenue for people with known ...
The rapid advancement of genome editing technologies has opened up new possibilities in the field of medicine. Nuclease-based techniques such as the CRISPR
Non-viral CRISPR/Cas gene editing in vitro and in vivo enabled by synthetic nanoparticle co-delivery of Cas9 mRNA and sgRNA Angew Chem Int Ed Engl, 56 (2017), pp. 1059-1063 CrossrefView in ScopusGoogle Scholar 28 Y. Li, T. Thambi, D.S. Lee Co-delivery of drugs and genes using pol...
in vitro transcribed gRNA and heterologous expressed Cas9, conferring nuclease activity in-vitro [10, 53], we tested (post FACS) for PBS lysis mediated editing activity and found an 2–3 fold increased editing when the PBS lysed protoplasts were left in PBS for 2 h at room temperature (...
Anti-HSV therapies are only suppressive because they do not eliminate latent HSV present in ganglionic neurons, the source of recurrent disease. We have developed a potentially curative approach against HSV infection, based on gene editing using HSV-spec
Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing. Nat. Biotechnol. 35, 1179 (2017). Article CAS Google Scholar Miller, J. B. et al. Non-viral CRISPR/Cas gene editing in vitro and in vivo enabled by synthetic nanoparticle co-delivery of ...