Best of all, CRISPR appears to be most efficient in microalgal genome editing based on the number of papers reported so far, even though it debuted last in the genome editing field (Table2). ZFN and TALEN have been used for mutagenesis ofChlamydomonasand a diatom, respectively [58,91], an...
RNAi in eukaryotic systems is not very reliable in the suppression of gene expression particularly in microalgae [110], and the type VI CRISPR systems may provide a better alternative. Different from any gnome editing techniques, the CRISPR system enables the delivery of preassembled Cas9 or Cpf1...
(2014), on the basis of gene expression analysis, this study describes heterogeneity in mast cells from various tissues of residence. Article CAS Google Scholar Abraham, S. N. & St John, A. L. Mast cell-orchestrated immunity to pathogens. Nat. Rev. Immunol. 10, 440–452 (2010). ...
The marks serve as sites for candidate cis-regulatory elements (cCREs) that may serve functional roles in regulating gene expression1. The project has been extended to model organisms, particularly the mouse. In the third phase of ENCODE, nearly a million and more than 300,000 cCRE ...
We also explore various methods for optimizing the function of MSC-EVs, including gene editing, modifying stem cell culture conditions and peptide modification. In addition, we discuss the therapeutic potentials of MSC-EVs, as well as the obstacles that currently impede their clinical utilization. ...
Chandrasekaran R, Rajiv P, Abd-Elsalam KA (2020) Carbon nanotubes: Plant gene delivery and genome editing. In: Abd-Elsalam K (ed) Carbon nanomaterials for agri-food and environmental applications. Elsevier, pp 279–296 Chapter Google Scholar Chang FP, Kuang LY, Huang CA, Jane WN, Hung ...
Cornerstones of CRISPR-Cas in drug discovery and therapy. We focus on the promises, pitfalls and hurdles of this revolutionary gene-editing technology, discuss key aspects of different CRISPR-Cas screening platforms and offer our perspectives on the best practices in genome engineering.doi:10.1038.....
Recent advances in gene editing have created a new generation of tools for gene therapy, i.e., molecular scissors to engineer the genome in a targeted and tailored fashion. To achieve this, different gene-editing tools were created, which are based on zinc-finger nucleases [161], transcription...
Its aim was to explore scientific, legal and ethical perspectives on the prospective use of human genome editing as a therapeutic intervention in disease (... Andy,Greenfield - 《Mammalian Genome Official Journal of the International Mammalian Genome Society》 被引量: 0发表: 2019年 Designing Our ...
In this Perspective, the authors suggest that different tumour microenvironments have ‘archetypal’ qualities across all cancers — characteristic and repeating collections of cells and gene-expression profiles at the level of the bulk tumour. They propose the existence of 12 dominant immune archetypes...