GDP-fucose transporter 1 (SLC35C1)GDP-fucose is used by fucosyltransferases in both the Golgi and endoplasmic reticulum (ER). As it is synthesized in the cytoplasm, transport into the lumen of the secretory pathway organelles is required. The first indication that GDP-fucose, like other ...
Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP- L -fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP- L -fucose over other nucleotide sugars in vitro , ...
Purification of the Rat Liver Golgi Membrane GDP-fucose Transporter All of the operations described below were performed at 4 °C. Step 1: Detergent Extraction A purified Golgi fraction was prepared from 250 g of frozen rat livers according to the procedure of Leelavathi et al. (17). This ...
32 Chen et al recently suggested that a multi–transmembrane protein termed SLC35C2 (gene CGI-15/Ovcov1) might transport GDP-fucose into the ER but expression of this protein reduced the Golgi-based fucosylation of Lex arguing against SLC35C2 to be the alternative transporter.33 Thus, the ...
GDP-fucose uptake into the Golgi apparatus during xyloglucan biosynthesis requires the activity of a transporter-like protein other than the UDP-glucose tr... The molecular mechanisms regulating hemicelluloses and pectin biosynthesis are poorly understood. An important question in this regard is how gly...
6-dehydratase and GDP-fucose transporter were knocked out in Chinese hamster ovary cells, a platform for biopharmaceutical protein production. By adapting ΔGMDΔGFT cells to serum-free medium and constructing suspension-cultured cells, we established host CHO cells with no detected fucosylated ...
Complementation cloning identifies CDG-IIc, a new type of congenital disorders of glycosylation, as a GDP-fucose transporter deficiency. Nat Genet 2001; 28: 73-76.Lubke T, Marquardt T, Etzioni A, Hartmann E, von Figura K, Korner C. Complementation cloning identifies CDG-IIc, a new type of...
WbgL: a novel bacterial ±1,2-fucosyltransferase for the synthesis of 2'-fucosyllactose.###FX enzyme and GDP-L-Fuc transporter expression in colorectal ca... expression profiles of the FX enzyme and GDP-L-Fuc Tr in a cohort of operated CRC patients to elucidate their role in 卤(1,6)fuc...
Hepatic GDP-fucose transporter SLC35C1 attenuates cholestatic liver injury and inflammation by inducing CEACAM1 N153 fucosylationBackground and Aims: Inflammatory response is crucial for bile acid (BA)-induced cholestatic liver injury, but molecular mechanisms remain to be elucidated. Solute Carrier ...
Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi