The soybean GDP-D-mannose pyrophosphorylase has an amino acid sequence of SEQ ID NO. 2, and the encoding gene GmGMP1 has a DNA sequence as shown in SEQ ID NO. 1. When the GmGMP1 transgenic arabidopsis thaliana is compared with wild-type arabidopsis thaliana, the total vitamin C content...
The GDP-D-mannose pyrophosphorylase (GMP) and microtubule severing enzyme KATANIN (KTN) are crucial for wood formation. Although functional identification has been performed in Arabidopsis, few comprehensive studies have been conducted in forest trees. In this study, we discove...
We created transgenic tobacco overexpressing peach GDP-d-mannose pyrophosphorylase (PpGMPH) and GDP-d-mannose-3′,5′-epimerase (PpGME), which are involved in the main l-ascorbate (Asc) biosynthetic pathway in plants. Despite an apparent increase in enzymatic activity in the PpGMPH-overexpressing...
GDP-mannose pyrophosphorylase gene (ManC) of Escherichia coli (E. coli) O157 was cloned and expressed as a highly soluble protein in E. coli BL21 (DE3). The enzyme was subsequently purified using hydrophobic and ion exchange chromatographies. ManC showed very broad substrate specificities for fo...
[1]. Elling L, et al. Expression, purification and characterization of recombinant phosphomannomutase and GDP-alpha-D-mannose pyrophosphorylase from Salmonella enterica, group B, for the synthesis of GDP-alpha-D-mannose from D-mannose. Glycobiology. 1996 Sep;6(6):591-7. ...
Expression, purification and characterization of recombinant phosphomannomutase and GDP-alpha-D-mannose pyrophosphorylase from Salmonella enterica, group B, for the synthesis of GDP-alpha-D-mannose from D-mannose. Glycobiology. 1996 Sep;6(6):591-7. GDP-α-D-mannose disodium物理化学性质 ...
GDP-d-mannose pyrophosphorylase catalyzes the production of GDP-d-mannose, an intermediate product in the plant ascorbic acid (AsA) biosynthetic pathway. This enzyme is a key regulatory target in AsA biosynthesis and is encoded by VITAMIN C DEFECTIVE 1 (VTC1) in the Arabidopsis thaliana genome....
GDP-d-mannose pyrophosphorylase (GMP; EC 2.7.7.13) was found to play a major role in the proposed AsA biosynthetic pathway in plants, considering that Arabidopsis vtc1-1 mutant with point mutation in this gene has a highly reduced AsA content. GMP cDNA was isolated from acerola fruits, ...
GDP-D-mannose pyrophosphorylasemolecular cloninggene expressionbin mappingGDP-D-mannose pyrophosphorylase (GMP, EC 2.7.7.22) catalyzes the synthesis of GDP-D-mannose and represents the first committed step in plant ascorbic acid biosynthesis. Using potato GMP cDNA sequence as a querying probe, 65 ...
GDP-d-mannose pyrophosphorylase (GMP) is one of the enzymes that highly expressed in acerola plants. A promoter assay suggests the presence of a new cis-element in the -1087 to -1083 bp sequence of the MgGMP promoter. Moreover, cis-elements, present in the -1080 to -600 bp sequence ...