D1.GDP-mannose4,6-dehydratase(GMD)assaydevelopmentandpHrateprofiles Thisprojectwillenablestudentstofunctionascompetentenzymologiststhroughthe developmentofanovelassayutilizedinthemeasurementofthekineticsofGMDwhile developingacomprehensiveappreciationoftheintricaciesofglycobiologyandan understandingoftranslationalresearchasa...
This work reports the biochemical and functional analysis of the Burkholderia cenocepacia J2315 bceN gene, encoding a protein with GDP-D-mannose 4,6-dehydratase enzyme activity (E.C.4.2.1.47). Data presented indicate that the protein is active when in the tetrameric form, catalyzing the convers...
Molecular Cloning of Human GDP-mannose 4,6-Dehydratase and Reconstitution of GDP-fucose Biosynthesis in Vitro We have cloned the cDNA encoding human GDP-mannose 4,6-dehydratase, the first enzyme in the pathway converting GDP-mannose to GDP-fucose. The message is ex... Sullivan,X F. - 《...
Structure of the MUR1 GDP-Mannose 4,6-Dehydratase from Arabidopsis thaliana: Implications for Ligand Binding and Specificity. GDP-D-mannose 4,6-dehydratase catalyzes the first step in the de novo synthesis of GDP-Lfucose, the activated form of L-fucose, which is a component of gly... Mul...