rGDF11补充老龄小鼠提高了卫星的再生能力细胞.近年来的研究发现,GDF11是有年轻的再生潜力的体液调节,并表明,恢复通过GDF11是老化卫星细胞功能与逆转积累的DNA损伤(Sinha等人,2014)。 TGF-β家族成员进行复杂的调控,由于许多蛋白质能够结合的配位体,并抑制其活动已经确定(Souza 等人,2008)。这些结合之一蛋白质是GDF...
故研究团队透过西方墨点法 (Western blot) 分析,发现年轻与老化鼠血液中的 GDF11 蛋白质含量有显著地差异,GDF11 在老化鼠血液中含量比较低,但与年轻鼠的血液循环互通后,老化鼠血液中的 GDF 11 含量又有回复的现象。所以研究团队决定单独将重组的 GDF11 (rebinant GDF11; rGDF11) 注入老化鼠的体内,测试是否也...
如本文所述的GDF11多肽可以包含通常存在于多肽和/或由 活生物体产生的蛋白质中的天然存在氨基酸,例如Ala (A)、Val (V)、Leu (L)、Ile (I)、Pro (P)、Phe(F)、Trp(W)、Met(M)、Gly(G)、Ser(S)、Thr(T)、Cys(C)、Tyr(Y)、Asn(N)、Gin(Q)、Asp (D)、Glu(E)、Lys(K)、Arg (R)和His(...
(r=-0.437,-0.430,-0.306,均P<0.05),与骨髓有核红细胞 比例,Ret百分比,MCV和骨髓CD235a的表达呈正相关(r=0.465,0.392,0.505,0.387,P<0.05),与MCHC和 MCH无相关性(均P >0.05).低危组MDS患者外周血GDF11浓度与骨髓CD235a的表达呈正相关(r=0.429,P<0.05),与外周血Hb,RBC,Ret 百分比,MCV,MCH,MCHC,...
Zeisberg M, Kalluri R. Cellular mechanisms of tissue fibrosis. 1. Common and organ-specific mechanisms associated with tissue fibrosis. Am J Phys Cell Phys. 2013;304:C216–25. CASGoogle Scholar Gabbiani G. The myofibroblast in wound healing and fibrocontractive diseases. J Pathol. 2003;200:500...
激活素与激活素IIA型(ActRIIA)或IIB型(ActRIIB)受体的结合诱导激活素I型受体的募集和磷酸化,然后磷酸化Smad2和Smad3细胞内信号蛋白[12]。GDF15上调是由于GDF11诱导SMAD2/3向GDF15启动子募集所致。抑制GDF15可以恢复食欲,但不能恢复GDF11诱导的肌肉质量损失,这需要阻断ActRII信号。关于GDF11诱导GDF15的机制,研究...
4r) displayed a change in PPR, suggesting that the reduced mIPSC frequency is not attributable to alterations in presynaptic release probability but may result from a change in the number of functional inhibitory synapses. In these electrophysiological experiments, the GFP- EN in control mice and ...
目的 探讨外源性予以重组生长转化因子11(r-GDF11)对SD大鼠动脉血管钙化的影响.方法 将SD大鼠分为钙化组、溶媒组、低浓度组、中浓度组、高浓度组5组,适应性饲养1周后,每日分别予以腹腔注射生理盐水、r-GDF11溶媒液及低、中、高浓度r-GDF11,持续7周后开始诱导钙化.诱导钙化7周后取各组大鼠血液,分离其...
R GAGAGGTCCACAGAGCTGATT R TGCACTTTGGTACTCTTGAAGTT Plin1 F CAAGCACCTCTGACAAGGTTC PLIN1 F TGTGCAATGCCTATGAGAAGG R GTTGGCGGCATATTCTGCTG R AGGGCGGGGATCTTTTCCT Plin2 F GACCTTGTGTCCTCCGCTTAT PLIN2 F ATGGCAGAGAACGGTGTGAAG2 R CAACCGCAATTTGTGGCTC R CAACTGCAATTTGCGGCTC Plin5 F TGTCCAGTG...
It was found that rGDF11 was able to reduce the levels of AGEs, protein oxidation and lipid peroxidation, and to slow down the accumulation of age-related histological markers, while rGDF8 was not. Moreover, rGDF11 significantly prevented the decrease in CAT, GPX and SOD activities, but ...