然而GCN4-motif在非贮藏蛋白种子特异性启动子的研究较少,AvBPPS作为非贮藏蛋白,表现出在种子中高表达特点和其产物表现出种子富集的特性[6,12],初步推断单个GCN4-motif在非贮藏蛋白种子特异性启动子中可以介导基因在种子中特异性表达且具有正调控的作用。因此,本研究重点关注AvBPPS启动子中的GCN4-motif,鉴定GCN4-...
然而GCN4-motif在非贮藏蛋白种子特异性启动子的研究较少,AvBPPS作为非贮藏蛋白,表现出在种子中高表达特点和其产物表现出种子富集的特性[6,12],初步推断单个GCN4-motif在非贮藏蛋白种子特异性启动子中可以介导基因在种子中特异性表达且具有正...
(1998) The GCN4 motif in a rice glutelin gene is essential for endosperm-specific gene ex- pression and is activated by Opaque-2 in transgenic rice plants. Plant J. (in press).Wu C, Suzuki A, Washida H, Takaiwa F (1998a) The GCN4 motif in a rice glutelin gene is essential for ...
SummaryThe GCN4 motif is conserved in a number of seed storage protein genes, and promoter fragments containing this motif have been shown to be involved in controlling seed‐specific expression of the genes studied. All genes encoding the rice seed storage protein glutelin contain the GCN4 motif...
GCN4motifREB.A bifactorial endosperm box (EB), which contains an endosperm motif (EM) and a GCN4 motif, was found in rice Wx promoter. EB was found in 5′ upstream region of many seed storage protein genes accounting for these genes expression exclusive in endosperm among various cereals....
对 AvBPPS 启动子截短片段中的 GCN4-motif 进行突变;构建由上述启动子驱动 β-葡萄糖苷酸 酶基因(β-glucuro-nidase gene,GUS)的重组表达载体,利用农杆菌介导法注射侵染本氏烟草叶片进行瞬时表达,验证启动子的 活性和 GCN4-motif 的功能.结果 克隆获得 365 bp 的 AlBPPS 启动子序列,3'端约 300 bp 的...
BLZ2, expressed in bacteria, binds specifically to the GCN4-like motif (GLM; 5′-GTGAGTCAT-3′) in a 43-base pair oligonucleotide derived from the promoter region of a Hor-2 gene (B1-hordein). This oligonucleotide also includes the prolamin box (PB; 5′-TGTAAAG-3′). Binding by ...
Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in B... 1999 Barley BLZ2, a seed-specific bZIP protein that interacts with BLZ1 in vivo and activates transcription from the GCN4-like ...
结果显示含有GCN4-motif的启动子比GCN4-motif突变或缺失的启动子显示出更强的活性,证明GCN4-motif对启动子活性起正调控作用。 4讨论 阳春砂萜类物质主要富集于种子团中,而BPPS作为砂仁药效萜类合成途径的关键酶,AvBPPS也相应地表现出种子特异性表达[6,12]。另外,AvBPPS在阳春砂种子团的表达量不仅显著高于其他的...
阳春砂海南砂GCN4-motif瞬时表达目的龙脑基二磷酸合酶(bornyl diphosphate synthase,BPPS)是砂仁药效萜类合成途径中的关键酶,获得海南砂AlBPPS的启动子并与阳春砂AvBPPS启动子进行调控元件和瞬时表达的比较,以期解析AvBPPS在种子中高表达的分子机制.方法通过FPNI-PCR的方法从海南砂gDNA中克隆AlBPPS的启动子,并与阳春...