然而GCN4-motif在非贮藏蛋白种子特异性启动子的研究较少,AvBPPS作为非贮藏蛋白,表现出在种子中高表达特点和其产物表现出种子富集的特性[6,12],初步推断单个GCN4-motif在非贮藏蛋白种子特异性启动子中可以介导基因在种子中特异性表达且具有正...
然而GCN4-motif在非贮藏蛋白种子特异性启动子的研究较少,AvBPPS作为非贮藏蛋白,表现出在种子中高表达特点和其产物表现出种子富集的特性[6,12],初步推断单个GCN4-motif在非贮藏蛋白种子特异性启动子中可以介导基因在种子中特异性表达且具有正调控的作用。因此,本研究重点关注AvBPPS启动子中的GCN4-motif,鉴定GCN4-...
然而GCN4-motif在非贮藏蛋白种子特异性启动子的研究较少,AvBPPS作为非贮藏蛋白,表现出在种子中高表达特点和其产物表现出种子富集的特性[6,12],初步推断单个GCN4-motif在非贮藏蛋白种子特异性启动子中可以介导基因在种子中特异性表达且具有正调控的作用。因此,本研究重点关注AvBPPS启动子中的GCN4-motif,鉴定GCN4-...
程世军,洪孟民,王宗阳.Rice bZIP protein,REB,interacts with GCN4 motif in promoter of Waxy gene[J]. Science in China(Series C:Life Sciences).2002(04)Cheng S, Wang Z, Hong M. 2002. Rice bZIP protein, REB, interacts with GCN4 motif in promoter of Waxy gene. Science in China Series C...
对 AvBPPS 启动子截短片段中的 GCN4-motif 进行突变;构建由上述启动子驱动 β-葡萄糖苷酸 酶基因(β-glucuro-nidase gene,GUS)的重组表达载体,利用农杆菌介导法注射侵染本氏烟草叶片进行瞬时表达,验证启动子的 活性和 GCN4-motif 的功能.结果 克隆获得 365 bp 的 AlBPPS 启动子序列,3'端约 300 bp 的...
SummaryThe GCN4 motif is conserved in a number of seed storage protein genes, and promoter fragments containing this motif have been shown to be involved in controlling seed‐specific expression of the genes studied. All genes encoding the rice seed storage protein glutelin contain the GCN4 motif...
The interaction between the two subunits forms a DNA binding domain made up of the basic motif region of each subunit. The DNA binding domain contains many basic residues that can interact with DNA. The structure of the GCN4–DNA cocrystal shows a dimer of two large subunits containing a...
Rap1 binding sites are preferentially located upstream of the Gcn4-binding sites in the upregulated targets, whereas they are located downstream in the downregulated targets (Supplementary Fig.8). In addition, the Gcn4 motif that we inferred from downregulated targets is shorter than the motif we...
Gcn4 recognizes a specific DNA-binding motifsequence designated the Gcn4-protein responsive element (GCRE), which is present in the promoter regions of its target genes. Gcn4 (31 kDa) targets include genes in every amino acid biosynthetic pathway except cysteine as well as genes encoding ...
Recently, the O2 protein has been shown to interact in vitro with another maize endosperm-specific factor (PBF) of the Dof (DNA-binding with onefinger) class that recognizes the PB motif in the −300 region of the 22-kDa zein promoter (14). Its barley orthologue (BPBF) transactivates ...