kinetics of H+,K+‐ATPaseconformations of the H+,K+‐ATPasefunctional residues of the H+,K+‐ATPasestructural model of the H+,K+‐ATPaseacid secretion and H+,K+‐ATPaseThe gastric H(+),K(+)-ATPase is responsible for gastric acid secretion. This ATPase is composed of two subunits, ...
The gastric parietal cell H,K-ATPase mediates the secretion of acid by an energy dependent, electroneutral exchange of intracellular H+against extracellular K+. Among the different known P-type ATPases, the α-subunits of the vertebrate H,K-ATPases and Na,K-ATPases share the greatest seque...
碳酸酐酶在壁细胞胞质中产生 H + 离子。这种酶催化 H2O 和 CO2 结合形成碳酸 (H2CO3),然后碳酸迅速分解为 H + 和 HCO3 −。二氧化碳 (CO2) 是碳酸合成所必需的,它从固有层中的毛细血管穿过基底膜进入细胞。 H +/K+-ATPase 质子泵将 H + 离子从细胞质穿过膜运输到小管腔。 同时,小管中的K+ 被运输到...
The effect of these four compounds was studied on vesicular preparations of the hog gastric H,K-ATPase under acid transporting conditions, in terms of the rate and level of inhibition of enzyme activity, acid transport, and the sites of labeling of the enzyme. The rate of inhibition of ATPase...
The mechanism of action and structure of the gastric H/K ATPase have been extensively reviewed in this volume and elsewhere. The protein is composed of two subunits, a catalytic 伪 and a 尾 subunit. The existence of the a subunit has been known for some time. The protein is 95 kDa and...
Preparation of gastric H+,K+-ATPase This chapter begins with a discussion on preparation of Hog Gastric H + , K + -ATPase. In this procedure, large yields of the membrane-bound H + , K + -ATP... Edd C Abon,WB Im,G Sachs - 《Methods in Enzymology》 被引量: 72发表: 1988年 ...
Projection maps obtained with frozen-hydrated two-dimensional crystals of the H+,K+-ATPase in these two states looked very similar, suggesting only small conformational changes during the transition from the E1 to the E1P·ADP state. This result differs from the X-ray crystal structures of the...
The H + ,K + -ATPase β-subunit is also important for blocking reversion of the transport cycle by stabi- lizinDgestphieteE2tPhecocnlofosermsiamtiiolnarwityithbeittswaemenintoheterpmriimnuarsy14,1s5t.ructures of the H + ,K + - and Na + ,K + -ATPase α...
H+ secretion by marginal cells of the stria vascularis was proposed based on observations of immunostaining of vH+-ATPase near the apical membrane of the epithelial cells [3, 7]. H+,K+-ATPase [8] and Na+,H+ exchangers [9–11] have been immunolocalized to strial marginal cells and ...
Gastric hydrogen-potassium ATPase (H+, K+-ATPase) is a heterodimeric protein which participates in the formation of hydrochloric acid. We cloned canine H+, K+-ATPase α and β subunit cDNAs from canine gastric cDNA libraries and the α subunit gene from a canine genomic library. The α subu...