TaqMan MGB probes have a nonfluorescent quencher at the 3´ end and a minor groove binder at the 3´ end that increases the melting temperature (Tm) of probe, allowing the use of shorter probes. For more information on MGB probes, please consult the following manual: "Primer Express v...
TaqMan MGB probes have a nonfluorescent quencher at the 3´ end and a minor groove binder at the 3´ end that increases the melting temperature (Tm) of probe, allowing the use of shorter probes. For more information on MGB probes, please consult the following manual: "Primer Express v...
Human GAPDH qPCR Primer Pair,即人GAPDH qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
Human GAPDH qPCR Primer Pair,即人GAPDH qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
England Biolabs, UK) for expression. Sequences ofGAPDHgene and primers used for cloning are given in Supplementary Table6. 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma) was used to induce expression of the gene in cells cultured at 37 °C. GAPDH protein was purified by Ni...
The pECFP Cerulean-tagged Apollo-NADP+ plasmid (addgene #71800) was used to clone the Apollo-NADP+ probe into the LeGO-iT2 vector backbone (addgene #27343) after restriction digest with BamH1 + BsrGI. (Forward primer: ACACAGGTGTCGTGACGCGGATGGCAGAGCAGGTGGCC, Reverse primer: GCTGGCGGCCG...
The kit comprises a PCR amplification buffering reaction system composed of specific primers and probe of GAPDH, heatproof DNA polymerase, dNTPs, template DNA, magnesium ions, a PCR buffer solution, ultrapure water, etc., and a standard ACTB template, wherein, the sequences of the primers ...
Figures S1–S7 and Tables S2–S4 Table S1. List of the 587 Probesets Corresponding to 524 Unique Genes Predicting Overall Survival of Patients with DLBCL Treated with R-CHOP (Analysis from Lenz et al., 2008), Related to Figure 1 Document S2. Article plus Supplemental Information...
Over-activation of AMPARs (α−amino-3-hydroxy-5-methylisoxazole-4-propionic acid subtype glutamate receptors) is implicated in excitotoxic neuronal death associated with acute brain insults, such as ischemic stroke. However, the specific molecular mec
probe in the appropriate volume of sterile 1X TE (1 mM Tris, 0.1 mM EDTA, pH 8.0) or sterile, nuclease-free H2O. Detailed instructions on how to calculate the proper volume of solution to add to your probe are provided in our online Tutorial document "Reconstituting and Diluting Primers ...